N甘氨酸对成年大鼠缺氧后处理心肌细胞活性氧水平影响.docVIP

N甘氨酸对成年大鼠缺氧后处理心肌细胞活性氧水平影响 　　摘要：目的 观察在再复氧前5min，活性氧清除剂N-（2-巯基丙酰基）-甘氨酸（MPG）对成年大鼠心肌细胞缺氧后处理活性氧水平表达的影响。方法 体外培养成年大鼠心肌细胞，建立心肌细胞缺氧后处理模型，然后将细胞随机分为4组，正常组（N组）、缺氧/复氧组（H/R组）、缺氧后处理组（HPO组）、缺氧-MPG-缺氧后处理组（H-M-HPO组），酶标法观察各组细胞内活性氧水平的变化。 结果 心肌细胞缺氧/复氧组活性氧水平高于正常组（P0.05），缺氧后处理组活性氧水平低于缺氧/复氧组（P0.05），缺氧-MPG-缺氧后处理组活性氧水平高于缺氧后处理组（P0.05）。 结论 缺氧后处理对成年大鼠心肌细胞缺氧/复氧损伤具有一定的保护作用；在再复氧前5min用MPG处理，使缺氧后处理对心肌细胞产生的保护作用降低。 　　关键词：N-二巯基丙酰甘氨酸 缺氧后处理 心肌细胞 活性氧 　　Abstract Objective To observe the effect of reactive oxygen scavenger N-（2-Mercaptopropionyl）glycine（MPG） on the expression of ROS level in adult rat myocardial cells after hypoxia post-processing， which proceed five minutes before reoxygenation. Methods Adult rat myocardial cells were vitro-cultured. Then， the hypoxia post-processing model of myocardial cells was established. The cultured cells were randomly divided into 4 groups， which were normal group（N）， hypoxia/ reoxygenation group （H/R）， hypoxia post-processing group（HPO），hypoxia-MPG- hypoxia post-processing group （H-M-HPO）. The variation of reactive oxygen level in the cells of each group was observed by enzyme linked immunosorbent assay（ELISA）. Results The reactive oxygen level in myocardial cells of H/R group is higher than that of N group （P0.05）. The reactive oxygen level in myocardial cells of HPO group was lower than that of H/R group. The reactive oxygen level in myocardial cells of H-M-HPO group is higher than that of HPO group （P0.05）. Conclusion Hypoxia post-processing has certain protective effect on adult rat myocardial cells during hypoxia/ reoxygenation injuries. The protective effect of hypoxia post-processing on myocardial cells was decreased with the treatment of MPG， processed five minutes before reoxygenation. 　　Key words N-（2-Mercaptopropionyl）glycine hypoxia post-processing myocardial cells reactive oxygen 　　活性氧（reactive oxygen，ROS） 作为呼吸链的副产品一直被认为是一种毒性分子，然而，随着研究的深入，人们发现ROS是细胞信号通路中的重要中介体，并认为低浓度的ROS是信号介导分子，可以调控多种信号传导通路，从而协调细胞适应环境的变化[1]。 ROS不但能激活抗氧化相关基因的表达还能激活其它转录因子如低氧诱导因子[2]。