IFNα―2b对白血病K562细胞增殖及FAK mRNA表达影响.docVIP

IFNα―2b对白血病K562细胞增殖及FAK mRNA表达影响 　　【摘要】 目的：研究重组人干扰素（IFNα-2b）对人慢性粒细胞白血病急变期细胞系K562细胞增殖及黏着斑激酶（FAK） mRNA表达的影响。方法：应用MTT法检测IFNα-2K562细胞的抑制率，采用RT-PCR技术检测各IFNα-2b浓度组对K562细胞FAK mRNA表达水平的影响。结果：增加IFNα-2b浓度及延长反应时间，除48 h和72 h外，抑制K562细胞增殖的比率逐渐升高；IFNα-2b作用48 h，浓度1万U/mL对抑制细胞增长的比率最高；随着IFNα-2b浓度增加，K562细胞的FAK mRNA表达增高，其中1万U/mL IFNα-2b实验组FAK mRNA表达水平最高，各组比较差异均有统计学意义（P0.05）。结论：白血病K562细胞可能存在FAK mRNA异常表达，IFNα-2b可能通过上调正常FAK mRNA的表达水平抑制K562细胞增殖。 　　【关键词】 IFNα-2b； K562细胞； FAK 　　Effects of Interferon α-2b on Cell Multiplication and FAK mRNA Expression in Leukemia K562 Cells/LIU Xiao-li，CHEN Ri-ling.//Medical Innovation of China，2016，13（17）：008-011 　　【Abstract】 Objective：To discuss recombinant human interferon （IFNα-2b） on human chronic myelogenous leukemia cell acute transformation phase cell line K562 cell proliferation and focal adhesion kinase （FAK） mRNA expression.Method：The inhibitory rate of IFNα-2b on K562 cells was detected by MTT method and the effect ofα-2b on the expression level of mRNA FAK in K562 cells was detected by RT-PCR technique.Result：In addition to 48 h and 72 h，the ratio of inhibiting K562 cell proliferation increased gradually by increasing IFNα-2b concentration and prolonging reaction time.IFNα-2b in 48 h and the concentration 10 thousands U/mL was the highest to inhibit cell growth.With the increase of the IFNα-2b concentration，FAK mRNA expression was increased by K562 cell，the highest expression level was experimental FAK mRNA of 10 thousands U/mL IFNα-2b，the differences were statistically significant of each groups（P0.05）.Conclusion：The abnormal expression of FAK mRNA in leukemia K562 cells，IFNα-2b may inhibit the proliferation of K562 cells by up regulating the expression level of normal mRNA FAK. 　　【Key words】 Interferonα-2b； K562 cells； Focal adhesion kinase 　　First-author’s address：The First Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 510405，China 　　doi：10.3969/j.issn.1674-4985.2016.17.002 　　白血病是造血系统的恶性疾病，在青少年儿童恶性肿瘤中白血病死亡率高