Toll样受体1基因多态性与华北地区汉族人群支气管哮喘关系研究.docVIP

Toll样受体1基因多态性与华北地区汉族人群支气管哮喘关系研究 　　[摘要] 目的 探讨Toll样受体1（TLR1）基因外显子3区的单核苷酸多态性（SNP）与华北地区汉族人群支气管哮喘之间的关系。 方法 选取山东省潍坊市人民医院（以下简称“我院”）呼吸科2014年1月～2015年1月收治的支气管哮喘患者100例为哮喘组，选取同期在我院健康查体100例为对照组，采集外周血并提取全基因组DNA。采用聚合酶链反应-高分辨率熔解曲线（PCR-HRM）技术和DNA测序技术检测TLR1外显子3区的基因多态性。比较哮喘组和对照组等位基因和基因型的分布差异。 结果 哮喘组与对照组TLR1基因SNP的rs5743596分布差异无统计学意义（P 0.05）。哮喘组与对照组等位基因C和T的分布频率差异无统计学意义（P 0.05）。 结论 TLR1基因rs5743596位点的多态性与支气管哮喘易感性之间关系不大。 　　[关键词] Toll样受体1；单核苷酸多态性；高分辨率熔解曲线技术；支气管哮喘 　　[中图分类号] R562.2+5 [文献标识码] A [文章编号] 1673-7210（2016）02（b）-0017-04 　　Association analysis between polymorphism of Toll-like receptors 1 and susceptibility to asthma of Han people in North China 　　XU Wenyuan1 DING Zhaolei2 XU Zhen3 SHENG Jianhui1 TAN Wei2 　　1.Weifang Medical University， Shandong Province， Weifang 261042， China； 2.Department of Respiratory Medicine， People′s Hospital of Weifang City， Shandong Province， Weifang 261041， China； 3.Department of Surgery， Maternal and Child Health Hospital of Ningyang County， Shandong Province， Ningyang 271400， China 　　[Abstract] Objective To investigate the association between single nucleotide polymorphisms （SNP） of excon-3 in Toll-like receptors 1 （TLR1） and susceptibility to asthma of Han people in North China. Methods 100 patients with asthma treated in Respiratory Department， People′s Hospital of Weifang City in Shandong Province （our hospital for short） from January 2014 to January 2015 were selected as asthma group， in the same period， 100 cases with kindergarden in our hospital were selected as control group. DNA samples were taken from their peripheral blood. Polymerase chain reaction-high resoution melting （PCR-HRM） technique and DNA sequencing technique were employed to detect the polymorphisms of excon-3 in TLR1. And meanwhile， distribution of allele and genotype between asthma group and control group were measured. Results There was no statistical difference in the distribution of TLR1-rs5743596 between asthma group and control group （P 0.05）. There were no statistic