VEGF在卵巢癌组织中表达及临床意义.docVIP

VEGF在卵巢癌组织中表达及临床意义 　　[摘要] 目的:探讨VEGF在卵巢癌组织中的表达及其与卵巢癌发生、发展的关系。方法:运用逆转录聚合酶链反应(RT-PCR)和免疫组织化学染色法检测VEGF在正常卵巢组织及卵巢癌组织中的表达情况。结果:卵巢癌组织中VEGF mRNA表达水平及蛋白阳性率均高于正常卵巢组织(P0.05),VEGF mRNA表达水平及蛋白阳性率与卵巢癌的浸润程度、临床分期和淋巴结转移有关(P0.05)。结论:卵巢癌组织中VEGF mRNA和蛋白含量均高于正常卵巢组织。VEGF可能参与了卵巢癌的发生、侵袭和转移。 　　[关键词] 卵巢癌;VEGF;逆转录聚合酶链反应;免疫组织化学 　　[中图分类号] R737.31[文献标识码]A [文章编号]1673-7210(2009)07(a)-009-03 　　 　　Expression and clinical significance of VEGF in ovarian carcinoma 　　YAN Linan1, LI Huiling2, WU Jian1, ZHANG Jisheng2 　　(1.Department of Clinical Laboratory, Female and Infant Hospital of Jiamusi, Jiamusi 154003, China; 2.Department of Clinical Laboratory, Jiamusi University, Jiamusi 154002, China) 　　[Abstract] Objective: To explore the expression of VEGF in ovarian carcinomas, and investigate the relationship with oncogenesis and progression of ovarian carcinoma. Methods: The mRNA and protein levels of VEGF in ovarian carcinoma and normal ovarian tissues were detected with reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: The mRNA level and positive rate of VEGF protein of ovarian carcinoma were significantly higher than that of normal ovarian tissues (P0.05). The VEGF mRNA level and positive rate were correlated with the infiltration degree, clinical stage and lymph node metastasis (P0.05). Conclusion: mRNA level of VEGF and protein of ovarian carcinoma are higher than that of normal ovarian tissues. It is possible that VEGF participates in the oncogenesis, transfer and invasion of ovarian carcinoma. 　　[Key words] Ovarian carcinoma; Vascular endothelial growth factor; RT-PCR; Immunohistochemistry 　　 　　卵巢癌是妇科恶性肿瘤中死亡率最高的一类肿瘤,卵巢癌诊断时70%已有盆腹腔转移,卵巢癌浸润、转移是造成卵巢癌治疗困难的主要原因。 　　肿瘤的生长、浸润和转移依赖于血管生成,血管内皮生长因子(vascular endothelial growth factor,VEGF)是目前已知作用最强的促血管生成因子之一,VEGF特异性作用于血管内皮细胞,通过促进内皮细胞增殖、增加血管通透性,以诱导肿瘤血管生成,在肿瘤的发生及发展中起关键作用[1]。 　　VEGF是一种多功能的细胞因子,能够诱导血管生成,导致肿瘤的生长、侵袭及远处转移。本研究旨在研究卵巢癌患者组织中VEGF的表达特点及其与卵巢癌临床病理参数的关系,探讨其对卵巢癌预后的影响以及与卵巢癌侵袭、转移的关系。 　　1 对象与方法 　　1.1 研究对象