miRNA―181a及其靶基因共济失调―毛细血管扩张突变基因在胃癌组织中表达及意义.docVIP

miRNA―181a及其靶基因共济失调―毛细血管扩张突变基因在胃癌组织中表达及意义 　　[摘要] 目的 观察胃癌组织中微小RNA-181a（miR-181a）与其靶基因共济失调-毛细血管扩张突变基因（ATM）的表达与异常情况，初步研究其意义。 方法 收集2009年4月～2011年12月广州市第一人民医院外科手术切除的胃癌组织标本9例，同时选取其癌旁非癌组织标本作为对照，分析胃癌组织中miR-181a与ATM蛋白表达的相关性。提取其蛋白质及总RNA，采用实时荧光定量PCR（qRT-PCR）检测标本的miR-181a，Western blot检测ATM蛋白。比较胃癌组织及癌旁非癌组织中miR-181a、ATM蛋白表达量。 结果 miR-181a在胃癌组织中的表达量为（1.981±1.800），miR-181a在邻近非癌组织中的表达量为（0.394±0.093）；灰度值检测：癌组织ATM蛋白为（0.2539±0.0046）；非癌组织为（0.5525±0.0660），癌及非癌组织中miR-181a、ATM蛋白表达量比较，差异均有高度统计学意义（P 0.01）；miR-181a与ATM蛋白表达呈负相关（r=-0.539，P 0.01）。 结论 miR-181a在胃癌组织中表达明显增高，ATM蛋白表达明显下降；究其原因可能是通过基因沉默机制miR-181a降低ATM蛋白表达量，从而在胃癌的发生及发展中起促进作用。 　　[关键词] 共济失调-毛细血管扩张突变基因；胃癌；微小RNA；miR-181a 　　[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-7210（2015）05（c）-0024-05 　　[Abstract] Objective To investigate the expression and significance of microRNA （miRNA）-181a （miR-181a） and its target gene ATM in gastric carcinoma tissues. Methods Tissues of gastric carcinoma and adjacent non-tumorous were collected respectively from 9 patients given surgical operation in Guangzhou First Peoples Hospital from April 2009 to December 2011. The total RNA and protein were extracted routinely， the miR-181a was detected by Real-time quantitative PCR， ATM protein was detected by Western blot. The expression of miR-181a and ATM protein between gastric carcinoma and adjacent non-tumorous were compared， and the correlation between miR-181a and ATM protein levels in gastric carcinoma was analyzed. Results In gastric carcinoma tissues， the expression of miR-181a and ATM protein were （1.981±1.800）， （0.2539±0.0046） respectively， whereas in the adjacent non-tumorous tissues， the expression of miR-181a and ATM protein were （0.394±0.093）， （0.5525±0.0660） respectively. The differences between the expression of miR-181a and ATM protein in these two tissues were statistically significant （P 0.01）. There was a negative correlation between miR-181a and ATM protein levels （r=-0.539， P 0.01）. Conclusion The expression of miR-181a is significantly up-regulat