TGFβ1对UVA照射皮肤成纤维细胞Ⅰ型,Ⅲ型胶原合成和表达影响.docVIP

TGFβ1对UVA照射皮肤成纤维细胞Ⅰ型,Ⅲ型胶原合成和表达影响 　　[摘要]目的：探讨转化生长因子-β1（tansforming gowth factor -beta 1，TGF-β1）对长波紫外线（ultraviolet A， UVA）照射皮肤成纤维细胞Ⅰ型、Ⅲ型胶原合成和表达的影响。 方法：通过MTT法检测TGF-β1干预后成纤维细胞增殖活性，选择UVA照射剂量为15J/cm2， 联免疫法（ELISA）测定不同剂量即TGF-β1小剂量组（UVA+TGF-β1 0.1ng/ml）、中剂量组（UVA+TGF-β1 1ng/ml）、大剂量组（UVA+TGF-β1 10ng/ml）处理后成纤维细胞清夜中Ⅰ型、Ⅲ型胶原含量，半定量RT－PCR检测成纤维细胞Ⅰ型、Ⅲ型胶原mRNA表达。结果：UVA照射体外培养的皮肤成纤维细胞,导致成纤维细胞增殖活性下降， 给予不同剂量TGF-β1干预后，成纤维细胞增殖活性与UVA照射组比较，明显提高；成纤维细胞上清液中Ⅰ型、Ⅲ型胶原含量增加，成纤维细胞内Ⅰ型、Ⅲ型胶原mRNA表达增强。 结论：TGF-β1可提高UVA照射体外培养成纤维细胞增殖活性；增加UVA照射体外培养的皮肤成纤维细胞Ⅰ型、Ⅲ型胶原含量，增强成纤维细胞Ⅰ型、Ⅲ型胶原mRNA表达，对皮肤成纤维细胞起保护作用。 　　[关键词]TGF-β1；UVA；皮肤成纤维细胞；Ⅰ型、Ⅲ型胶原 　　[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455（2009）03-0338-04 　　 　　The effects of TGF-β1 on the synthesis and expression of Ⅰ，Ⅲ collagen in skin fibroblasts after UVA irradiation in vitro 　　WANG Ji-hua,LIU Yin,HE Yong-jing，DU Yong-gui，ZHAO Ya-nan 　　（Department of Plastic Surgery，the Second Affiliated Hospital of Kunming Medical College,Kunming,650101,Yunnan） 　　Abstract: Objective To investigate the effects of TGF-β1 on the synthesis and expression of Ⅰ,Ⅲ collagen in skin fibroblasts after UVA irradiation in vitro. Methods Methyl thiazolyl tetrazolium （MTT） was used to test cell proliferation activity,and Enzyme-linked immunosorbent assay (ELISA) was used to determine the content of Ⅰ,Ⅲ collagen in the supernatant of skin fibroblasts after adding different dose of TGF-β1( low dose 0.1 ng / ml,medium dose1.0 ng / ml、 high dose 10.0 ng / ml) and the fibroblasts were irradiated by UVA15 J/cm2 . The mRNA expression of Ⅰ,Ⅲ collagen by semi-quantitative( RT-PCR) Results UVA irradiation decreasesed the fibroblasts OD value.The fibroblasts OD value was markedly increased,compared to UVA irradiation group. With the dose of TGF-β1 increased，the experiment showed that large, and medium dose group OD values were markedly higher than that of the UVA irradiation group. Ⅰ,Ⅲ collagen protein content increased with the dose of TGF-β1 increased ，and the mRNA expression of Ⅰ,Ⅲ collagen also increase