SRC―1对异位内膜雌激素调控CXCL12表达影响.docVIP

SRC―1对异位内膜雌激素调控CXCL12表达影响 　　[摘要] 目的 研究子宫内膜异位症患者的异位内膜中类固醇激素受体辅活化子-1（SRC-1）和趋化因子配体12（CXCL12）的表达水平，从而了解SRC-1对异位子宫内膜雌激素（E2）调控的CXCL12表达的影响。方法 2014年9月―2016年9月，方便选择在苏州大学附属第一医院妇科因内异症接受手术治疗，术后标本证实为内异症的15例患者的异位内膜及同期放置宫内节育器的无内异症且月经周期正常的健康妇女10名的正常内膜，应用实时荧光定量RT-PCR方法比较正常子宫内膜和异位子宫内膜在月经的增生期和分泌期SRC-1和CXCL12mRNA表达水平。ELISA检测沉默异位内膜SRC-1的表达对雌激素诱导异位内膜基质细胞表达CXCL12的影响。结果 正常子宫内膜基质细胞中SRC-1和CXCL12的表达呈现周期性变化，而异位内膜这两种因子的表达并没有周期性的改变。异位内膜SRC-1和CXCL12mRNA的表达与正常内膜相比明显升高。沉默异位内膜SRC-1表达后，E2诱导的CXCL12表达下降50.04%（P0.05）。 结论 类固醇激素受体辅活化子调控异位内膜表达CXCL12的作用中，SRC-1是雌激素的主要辅激活化子。 　　[关键词] 甾体激素受体辅活化子-1；趋化因子配体12；子宫内膜异位症 　　[中图分类号] R737.9 [文献标识码] A [文章编号] 1674-0742（2016）12（c）-0034-03 　　[Abstract] Objective To observe the expression of steroid coactivator-1（SRC-1） and steroid-induced CXCL12 in endometriosis （EMS） and to explore the roles of SRC-1in the steroid-induced CXCL12 expression in EMS. Methods Convenient selection from September 2014 to September 2016，15 endometriosis cases undergoing surgery in The First Affiliated Hospital of Soochow University were enrolled in this study.Their ectopic endometriosis were from ovarian endometriomata which were identified pathologically. The 10 normal endometrium were acquired from the healthy women with normal cycle. Quantitative real-time polymerase chain reaction was used to quantify the mRNA levels of SRC-1 and CXCL12 during the menstrual cycle. ELISA was adopted to analyze the steroids-induced CXCL12 expression before and after the SRC-1 was silenced by siRNA. Results The SRC-1 and CXCL12 showed marked cyclic differences in normal endometrium. There were no periodic variation in the expression of SRC-1and CXCL12 in ectopic endometrium throughout the menstrual cycle. The SRC-1 and CXCL12 of ectopic endometrium were significantly higher than that of normal endometrium. Silencing of SRC-1 significantly reduced the E2-induced CXCL12 expression to 50.04%（P0.05）. Conclusion SRC-1 is the major coactivator of E2-induced CXCL12 expression in EMS. 　　[Key words] Steroid recept