三羟基异黄酮对肝纤维化大鼠血清和肝组织中TGFβ1表达影响.docVIP

三羟基异黄酮对肝纤维化大鼠血清和肝组织中TGF-β1表达的影响 　　【摘要】 目的 观察4,5, 7-三羟基异黄酮(Genistein)对四氯化碳(CCl4)诱导的肝纤维化大鼠的防治作用及对转化生长因子β1(TGF-β1)表达的影响。方法 采用CCl4造成大鼠肝纤维化模型,在实验的第10周末采血和取肝组织,检测血清中ALT、AST、TGF-β1和Ⅰ型胶原,HE染色法观察肝组织改变, 免疫组织化学法检测肝组织TGF-β1表达。结果 血清学检测显示Genistein能明显降低肝纤维化大鼠ALT、AST、TGF-β1和Ⅰ型胶原水平,病理组织学观察发现Genistein能明显改善肝纤维化大鼠肝组织结构和肝纤维化, 免疫组织化学检测表明肝组织TGF-β1表达量与模型组比较显著降低。结论 Genistein对CCl4诱导的肝纤维化大鼠有较好的保护作用, 对TGF-β1表达的影响可能是其作用机制之一。 　　【关键词】 三羟基异黄酮;肝纤维化;TGF-β1 　　 　　Effect of Genistein on TGF-β1 in plasma and hepatic tissue of CCl4-induced hepatic fibrosis rats 　　 　　LIAO Ming.Guangxi Medical University, Nanning 530021, China 　　 　　【Abstract】 Objective To investigate the antifibrotic effect of 4, 5, 7-Genistein on CCl4-induced liver fibrosis in rats and expression of TGF-β1. Methods A hepatic fibrosismodel was induced by CCl4.The levels of alanine transaminase (ALT), aspartic transaminase (AST), TGF-β1 and type-Ⅰcollagen in plasma were detected by chemical kit. The deposition of collagen was observed with HE staining. The expression of TGF-β1 in hepatic tissue was observed by immuno-histochemistry. Results The concentration of serum ALT, AST, TGF-β1 and type-Ⅰcollagen was significantly reduced in the middle and high dose treated groups compared with that of model group. A significant reduction of collagen deposition and rearrangement in Genistein -treated group was displayed in histopathological changes. The expression of TGF-β1 was considerably decreased in the treated animals. Conclusion Genistein has an obvious protective effect on model rats, and remarkably inhibits TGF-β1 expression in plasma and liver tissue which is thought to be one possible antifibrogenic mechanism. 　　【Key words】 Genistein; Hepatic fibrosis; TGF-β 　　 　　? 　　基金项目:广西地方性高发疾病防治研究重点实验室开放基金(项目编号:桂科能0842009-k5) 　　作者单位:530021 广西医科大学 　　 　　肝纤维化是各种原因引起的慢性肝损伤所共有的病理改变,其特征是以胶原为主的细胞外基质(extracellular matrix, ECM)在肝内过量沉积。肝组织胶原沉积曾被认为是有害的和不可逆转的过程,但近来大量的临床和实验室证据均表明早期肝纤维化有逆转的可能[1,2] 。转化生长因子β1(transforming growth factor β1,TGF-β1)是强有力的致纤