三七总皂苷对子宫内膜癌Ishikawa和HEC―1A细胞增殖侵袭和凋亡影响.docVIP

三七总皂苷对子宫内膜癌Ishikawa和HEC―1A细胞增殖、侵袭和凋亡的影响 　　[摘要] 目的 探讨三七总皂苷（PNS）对子宫内膜癌Ishikawa和HEC-1A细胞增殖、侵袭和凋亡的影响及其作用机制。 方法 应用不同浓度的PNS分别作用于Ishikawa和HEC-1A细胞24、48、72 h，采用MTT法检测细胞增殖率；Transwell检测细胞侵袭能力；流式细胞仪检测细胞凋亡水平；采用RT-PCR和Western blot分别检测VEGF、PI3K、AKT的mRNA和蛋白表达水平。 结果 PNS可以显著抑制Ishikawa和HEC-1A细胞的增殖。与control组比较，PNS处理的Ishikawa和HEC-1A细胞侵袭显著下降（P 0.05），细胞凋亡率明显升高（P 0.05），VEGF和PI3K的表达水平下降（P 0.05）。 结论 PNS能够抑制Ishikawa和HEC-1A细胞的增殖和侵袭，诱导细胞凋亡，其过程可能与VEGF/PI3K/AKT信号通路的抑制有关。 　　[关键词] 三七总皂苷；子宫内膜癌；血管内皮生长因子；PI3K/AKT信号通路 　　[中图分类号] R737.33 [文献标识码] A [文章编号] 1673-7210（2016）05（b）-0013-04 　　[Abstract] Objective To investigate the effects of Panaxnotoginsengsaponins （PNS） on the cell proliferation， invasion and apoptosis in endometrial cancercell line Ishikawa and HEC-1A and its underlying mechanism. Methods Ishikawa and HEC-1A cells were treated with different density of PNS for 24， 48， 72 h. The MTT method was used to detect the cell proliferation rate after Ishikawa and HEC-1A； cell invasion ability was detected by Transwell method； cell apoptosis was measured by flow cytometry； the mRNA and protein expression level of VEGF， PI3K and AKT were detected by RT-PCR and Western blot， respectively. Results PNS could significantly inhibited the cell proliferation of Ishikawa and HEC-1A cells. Compared with control group， the cell invasion ability of Ishikawa and HEC-1A apparently decreased （P 0.05）， and the cell apoptosis were elevated with PNS treatment （P 0.05）， the expression of VEGF and PI3K decreased with PNS treatment （P 0.05）. Conclusion PNS inhibits the proliferation， invasion and induces apoptosis in Ishikawaand HEC-1A cells， which may be related to the inhibition of VEGF/PI3K/AKT signaling pathway. 　　[Key words] Panaxnotoginsengsaponins； Endometrial cancer； Vascular endothelial growth factor； PI3K/AKT 　　子宫内膜癌是三大妇科恶性肿瘤之一[1-2]。目前子宫内膜癌的治疗方法较多，以手术和放疗为主，辅助化学治疗或激素治疗，然而化学及激素治疗存在明显的复发症和毒副作用[3-4]。因此，寻找高效、安全、使用范围广的子宫内膜癌治疗新药是现今的研究热点。三七总皂苷（panaxnotoginsengsaponins，PNS）三七的主要有效成分，具有扩张血管、降低血脂和抗炎等药理作用[5-6]。