三七总皂苷提取分离纯化技术研究进展.docVIP

三七总皂苷提取、分离纯化技术的研究进展 　　[摘要]三七总皂苷（Panax notoginseng saponins，PNS）作为三七（Panax notoginseng）的主要活性成分，具有多种药理作用和临床应用，广泛应用于药品、保健品、日化品等行业。随着PNS的需求量日益上升，PNS的提取、分离纯化技术显得尤为重要。目前，PNS的提取、分离纯化技术的研究甚多，综合考虑其提取率、经济效益及规模化生产等因素，常采用乙醇回流提取，经大孔吸附树脂分离纯化，部分联合酶解技术和醇沉技术制备PNS。本文将对近年来实验研究中常用到的PNS提取、分离纯化技术进行综述，为PNS的进一步研究及工业生产等提供参考。 　　[关键词]三七；三七总皂苷；提取技术；分离纯化技术 　　[中图分类号] R283.3 [文献标识码] A [文章编号] 1674-4721（2016）07（c）-0019-05 　　[Abstract]Panax notoginseng saponins（PNS），as the main active ingredient of Panax notoginseng，which has a variety of pharmacological effects and clinical applications，and it is widely used in medicine，health products，cosmetic products and other industries.With the rising demand for PNS，extracting and separating and purifying technologies of PNS are particularly important.Currently，the studying on extractive， separative and purificative technology for PNS are especially more，considering its extraction rate，economy，large-scale production and other factors，using one of the first and formost method，which is the frequently-use method that of extracting and separating and purifying PNS from Panax notoginseng，firstly adopts the technique of ethanol refluxing extraction，then uses the method of macroporous resin adsorption to separate and purify PNS，and parts of them also combine with enzymolysis approach and alcohol precipitation approach to prepare PNS.In this paper，the commonly used techniques in laboratory research，which are the methods of extracting and separating and purifying PNS from medicinal material Panax notoginseng in recent years，are reviewed to provide the reference to the further studying on PNS and industrial production. 　　[Key words]Panax notoginseng；Panax notoginseng saponins；Extractive technique；Separative and purificative technique 　　三七为五加科人参属植物三七（Panax notoginseng（Burk.）F.H.Chen）的干燥根，又称田七，古代称其为金不换，是我国传统名贵中药材，主产于我国云南文山和广西，目前多用其栽培品。其在《跌损妙方》《医门秘旨》《本草纲目》中均有详细记载，有止血、活血化瘀、消肿止痛等功效，其活性成分主要是三七总皂苷（Panax notoginseng saponins，PNS）[1]。PNS具有滋补强身、调节免疫等作用，主要用于心、脑血管及循环系统。现代药理研