乙型肝炎病毒核酸与同义密码子遗传特点分析.docVIP

乙型肝炎病毒核酸与同义密码子的遗传特点分析 　　摘 要：乙型肝炎病毒是一种严重威胁公共健康的病毒性传染病。本文分析了乙型肝炎病毒基因组中开放阅读框中所有编码阅读框序列的遗传特征，于此同时我们还分析了病毒同义密码子使用模式与不同模式生物密码子使用模式的差异性。结果发现，乙型肝炎病毒基因对同义密码子使用的频率是不均一的，其中TCC，TCT，AGT，AGA是过量使用的，而ACC，CGG，GCC，ATA，CGT，CCC，TCG和GGT的使用频率极低。通过分析病毒与不同模式生物的密码子使用模式的差别，发现病毒自身的核苷酸变异和宿主对其自然选择的遗传压力是乙型肝炎病毒在遗传进化方面达到了一个相对平衡的局面。 　　关键词：乙型肝炎病毒；基因组；同义密码子；进化 　　Abstract： Hepatitis B virus （HBV） is the causative agent of an important disease for human beings. The synonymous codon usage pattern of open reading frame of HBV was analyzed by the relative synonymous codon usage value based on the principle component analysis， viral synonymous codon usage distribution compared with those of the five organisms （E. coli， mouse， human， gorilla gorilla and pan troglodytes）， and their overall codon usage bias was estimated by the codon adaptation index. The genetic diversity of 58 strains of HBV indicates that the compositional constraint deriving from nucleotide composition plays an important role in shaping the synonymous codon usage pattern of HBV. It is interesting that the four codons （TCC， TCT and AGT for Ser and AGA for Arg） of HBV are over-represented usage and the eight codons （ACC for Thr， CGG for Arg， GCC for Ala， ATA for Ile， CGT for Arg， CCC for Pro， TCG for Ser and GGT for Gly） are under-represented usage， and these codons usage under the strong translation selection probably serves as a potential genetic marker for HBV. Based on the codon usage bias of HBV， a balance of mutation pressure from virus itself and translation selection from the host acts on the synonymous codon usage patterns of HBV. The candidate systems for expression proteins encoded with the codon usage profiles of HBV are human， mouse and gorilla gorilla， while the overall codon usages of the cells deriving from E. coli. and pan troglodytes are not adapted to the synonymous codon usage distribution of this virus to some degree. These conclusions could not only offer an insight into the synonymous codon usage pattern of HBV， b