中药仙鹤复方水提取物对肝癌H22细胞增殖与凋亡影响及其作用机制.docVIP

中药仙鹤复方水提取物对肝癌H22细胞增殖与凋亡的影响及其作用机制 　　摘要： 　　目的：观察中药仙鹤复方水提取物（aqueous extract of Xian-he Compound，XHC）对肝癌H22细胞增殖与凋亡的影响及对炎症因子COX-2的影响。方法：采用四甲基偶氮唑盐（MTT）比色法检测不同浓度的H22 肝癌细胞增殖情况；Hoechst 33342染色观察细胞形态学变化；western blot检测COX-2蛋白质表达；RT-PCR法检测COX-2mRNA水平表达的变化。结果：XHC各剂量组较空白对照组对肝癌H22细胞均有明显的抑制作用，以作用24h后的1.8g/mLXHC组作用最显著，抑制率为71.25%±0.32%；与空白对照组相比，LPS诱导后加1.8g/mLXHC组有较显著的凋亡率，凋亡率由1.91%±0.45%增加至8.53%±0.13%；同样作用条件下与空白对照组及阳性对照组相比COX-2蛋白质及mRNA的表达明显下调。 结论：XHC的抑瘤作用与下调COX-2蛋白质及mRNA的表达有密切关系。 　　关键词：中药仙鹤复方水提取物；肝癌H22细胞株；COX-2 　　To study the antitumor mechanism of aqueous extract of Xian-he compound 　　to H22 hepatoma cells transplanted murine Xue-wen Yuan 1 Yu-jie Chen 1 Xiao-yan Zhang2 Hui-qin Huang 1 （1.The pharmacy department of Third Peoples Hospital of Huizhou，2. The pharmacy department of He-nan-an Hospital of Huizhou， Huizhou， Guangdong 516000） 　　Abstract： 　　Objective： To investigate the effect of XHC on the proliferation and apoptosis as well as the expression to H22 hepatoma cells in vitro.Methods： The inhibitory effect of different conc entrations of XHC on H22 cells was examined by methyl thiazolyl tetrazo lium（ MTT） assay； the H22 cell apoptosis after each test is analysed by Hoechst 33342 staining； the expression of COX protein and mRNA was determined by western blot and RT-PCR individually.Results： each dose group of XHC compared with the control group can significantly inhibit the proliferation of H22 cells，specially，the 1.8g/mLXHC group after 24h is the best at 71.25% ± 0.32%. and also， the LPS-induced plus 1.8g/mLXHC group had more significant apoptosis rate from 1.91% ± 0.45% to 8.53% ± 0.13%； at this conditions， the expression of COX-2 protein and mRNA was significantly reduced compared with the control group and positive group.Conclusion：The XHC has a better anti-tumor effect in animals， which may be closely related with the COX-2. 　　Key words：XHC； H22 hepatoma cell line； COX-2 　　【中图分类号】 　　R28 【文献标识码】B 【文章编号】1002-3763（2014）06-0150-02 　　中药仙鹤复方为临床经验方化裁而成。中医学认为由于人体正气内虚，脏腑