人精子顶体内透明质酸酶与男性不育关系研究.docVIP

人精子顶体内透明质酸酶与男性不育关系的研究 　　【摘要】 目的：研究人精子顶体内透明酸酶（HYD）对男性生育力的影响。方法：收集不育男性精液44例，根据精液常规检查结果分为不育A、B、C、D四组；正常生育男性精液26例为正常生育组。采用改良透明质酸钠-明胶（NaHY-Gelatin）底物膜法检测精子顶体内HYD活性强度、阳性率及反应时间，研究精子形态与HYD活性强弱的关系。同时采用固定明胶底膜法检测精子顶体内蛋白酶（Acrosin），并对两种酶检测结果作直线相关分析，精液分析仪进行精液质量分析。结果：正常生育组HYD反应平均阳性率为（70.84±19.20）%，HYD活性亮区平均直径为（38.17±16.41）μm；不育组总体HYD分别为（37.01±35.13）%、（23.05±17.32）μm；不育A组分别为（60.02±29.10）%、（26.92±17.21）μm；不育B组分别为（16.11±4.64）%、（6.90±2.00）μm；不育C组分别为（29.11±11.85）%、（8.22±3.37）μm；不育D组分别为（53.04±28.60）%、（31.40±18.60）μm。正常生育组HYD反应阳性率和HYD活性强度与不育B、C组比较差异均有统计学意义（P0.05）。Acrosin活性检测的相应数据证明人精子HYD活性与Acrosin活性呈显著正相关（r=0.810）。结论：精子内HYD活性低下是导致男性不育的重要原因之一；检测精子顶体内HYD可作为临床评价精子功能的有效指标。 　　【关键词】 精子； 透明质酸葡萄糖胺酶； 男性； 不育 　　【Abstract】 Objective： To investigate the effect of human sperm hyaluronidase on male fertile ability. Method： 44 semen samples of infertile patients were collected， and they were divided into 4 groups （A， B， C， D） according to the results of semen routine examine； 26 samples were collected from normal fertile men as control group. The improved sodium hyaluronate - gelatin substrate film technique was used to determine the acrosomal hyaluronidase active intension， positive reaction rates and the enzyme reaction time in single sperm， the relationship between sperm morphology and hyaluronidase activity were studied； at the same time， acrosin activity was determined by fixed gelatin substrate film technique， and the correlationship of the two acrosomal enzymes was analyzed. CASA was made by sperm quality analyzer . Result： In the control group， the mean positive reaction rate of the sperm hyaluronidase was （70.84±19.20）%， and the mean diameters of hyaluronidase reaction area was （38.17±16.41）?m. The infertile groups were （37.01±35.13）%， （23.05±17.32）?m . In the experimental groups A， B， C and D， the mean positive reaction of hyaluronidase were （60.02±29.10）%， （16.11±4.64）%，（29.11±11.85）% and （53.04±28.60）%； the mean diameters of hyaluronidase reaction area were （26.92±17.21）?m， （6.90±2.00）?m