从Wntβ―catenin信号通路探讨透骨消痛胶囊对关节软骨保护作用吴追乐.docVIP

从Wntβ―catenin信号通路探讨透骨消痛胶囊对关节软骨的保护作用吴追乐 　　【摘 要】目的：从Wnt/β-catenin信号通路观察透骨消痛胶囊对大鼠膝骨关节炎关节软骨的保护作用。方法：60只健康清洁级2月龄雄性SD大鼠，随机分为正常对照组、模型对照组和透骨消痛胶囊组，每组20只。模型对照组和透骨消痛胶囊组采用质4%木瓜蛋白酶双膝关节腔注射，成功复制骨关节炎模型后，透骨消痛胶囊组给予透骨消痛胶囊287.5 mg?kg-1，正常对照组和模型对照组给予相同剂量的生理盐水。给药2个月后，采用Western blot法观察各组关节软骨中含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-5（ADAMTS-5）、Wnt-4、β-catenin和基质金属蛋白酶-13（MMP-13）的表达。结果：与正常对照组比较，模型对照组ADAMTS-5、Wnt 4、β-catenin和MMP-13表达升高（P 0.05）；与模型对照组比较，透骨消痛胶囊组ADAMTS-5、Wnt 4、β-catenin和MMP-13表达降低（P 0.05）。结论：透骨消痛胶囊对关节软骨的保护作用与其降低Wnt/β-catenin信号通路表达相关。 　　【关键词】 骨关节炎，膝；透骨消痛胶囊；Wnt/β-catenin信号通路；体内实验；大鼠 　　doi：10.3969/j.issn.2095-4174.2015.12.001 　　【ABSTRACT】 Objective：To investigate the protective effect of Tougu Xiaotong Jiaonang（透骨消痛胶囊） 　　on knee articular cartilage of rats from Wnt/β-catenin signaling pathway.Methods：Sixty healthy two-month male SD rats were randomly divided into a normal control group，a model control group and a Tougu Xiaotong Jiaonang group （TXJ group），20 rats in each.The model control group and the TXJ group were given double knee joint cavity injection with 4% papain.After the osteoarthritis models were successfully established，the TXJ group was given Tougu Xiaotong Jiaonang 287.5 mg?kg-1，while the normal control group and the model control group were given the same dose of physiological saline.After 2 months of administration，the Western blot method was used to observe the expressions of ADAMTS-5，Wnt-4， 　　β-catenin and MMP-13 in articular cartilage of three groups.Results：Compared with the normal control group，the expressions of ADAMTS-5，Wnt-4，β-catenin and MMP-13 in the model control group increased 　　（P 0.05），while compared with the model group，the expressions of ADAMTS-5，Wnt-4，β-catenin and MMP-13 　　in the TXJ group decreased（P 0.05）.Conclusion：The protective effect of Tougu Xiaotong Jiaonang on articular cartilage is correlated with the reduction of the Wnt/β-catenin signaling pathway expression. 　　【Keywords】 osteoarthritis，knee； 　　Tougu Xiaotong Jiaonang（透骨消痛胶囊）； 　　Wnt/β-