初步探讨酥油茶对SD大鼠肝脏影响及作用机制多.docVIP

初步探讨酥油茶对SD大鼠肝脏的影响及作用机制多 　　摘要：目的 研究短期内酥油茶对SD大鼠肝脏的影响。 方法 将96只SPF级SD大鼠随机分成8组（空白组、高脂组、茶水组、盐水组、酥油高组、酥油低组、酥油茶高组、酥油茶低组），每组含雌雄鼠各6只。每日上午同一时间点每只大鼠给予特定溶液灌胃（3.6ml/200g），10d后取肝脏放入福尔马林溶液中备检，采用HE染色观察其结构变化、免疫组化观察低密度脂蛋白受体（LDL-R）在肝组织中的表达并用Image-proplus6.0软件对其阳性表达的细胞进行计数。结果 同空白组相比，酥油茶高、低两组的肝脏形态结构未见明显改变。高脂组及酥油高组肝细胞胞质内可见脂肪空泡、细胞体积增大、肝索间隙变窄；肝细胞LDL-R阳性表达结果显示，酥油茶高、低组及酥油低组的肝细胞LDL-R阳性表达数显著高于空白组（P0.01），高脂组的肝细胞LDL-R阳性表达数显著低于其余7组（P0.01）。结论 短期内酥油茶中酥油及茶成分的共同作用使SD大鼠肝细胞LDL-R呈阳性高表达状态，促进肝脏对血浆LDL的清除，进而调节血脂代谢。 　　关键词：酥油茶；SD大鼠；肝脏；脂质代谢 　　Influence of Butter Tea on the Liver of SD Rats and Its Mechanisms 　　Duo ji zhuo ma1，CUI Chao-ying1，QI Zhen-nan2，Gong ga lan zi1，JIANG Wen-hua3 　　（1.Department of Plateau Medical Research Center， Medical College of Tibet University， Lhasa 850000，Tibet，China；2.Department of General Practice，Peking University First Hospital ，Beijing 100034，China；3.Department of Histology and Embryology，College of Basic Medical Sciences，Jilin University，Changchun 130000，Jilin，China） 　　Abstract：Objective This study focuses on the influence of butter tea on SD rats liver. Methods We carried out the research by choosing 96 healthy SD rats （200g±20g）， half male and half female， and divided them into 8 groups which we called the blank group， high lipid group， tea water group，salt water group， high butter，s concentration group，low butter，s concentration group， high butter tea，s concentration group and low butter tea，s concentration group respectively. There are 12 rats in each group. We gave the SD rats intragastric administration of specific butter tea （3.6ml for each） at the same time every morning and weighed them every 10 days. The structure of liver is observed by means of HE staining. We detected low density lipoprotein receptor （LDL-R） by the immunohistochemical methods，and Observed in the tissue of liver and the positive expression cells count by Image - pro plus6.0 software. The obtained data were represented by average numbers plus standard deviation and processed by SPSS Statistic