叶绿素仪在河南平原烟区烤烟上应用.docVIP

叶绿素仪在河南平原烟区烤烟上应用 　　摘要：为了研究SPAD-502叶绿素仪在河南平原烟区烤烟叶片上的应用方法，选取河南许昌烟区主栽品种中烟100和豫烟系列新品种豫烟9号作为试验材料进行研究。结果显示，在一天中随着光照度的变化，两个烤烟品种叶片的SPAD值也有相应的变化。综合考虑测量的稳定性和可操作性等因素，上午8：00-10：00是测定的最佳时间段。用酒精棉擦拭测点和SPAD仪测试窗口，发现清洁后测得的结果有显著变化。分别在团棵期、旺长期和成熟期等烟叶生长发育期内对两个烤烟品种不同叶位的SPAD值进行分析发现，顶部完全展开叶的第3片叶测定结果相较于其他叶位变异系数较低，测定结果稳定；将顶3叶叶面分为10个测定位点进行分析发现，主脉中部左右两侧位点均值的变异系数和代表系数都较低，结果的稳定性和代表性为最优。 　　关键词：SPAD叶绿素仪；烤烟；河南平原烟区 　　中图分类号：S572；Q945.1 文献标识码：A 文章编号：0439-8114（2018）03-0051-06 　　DOI：10.14088/j.cnki.issn0439-8114.2018.03.013 　　Abstract： In order to study the method of SPAD-502 chlorophyll meter application in flue-cured tobacco of Henan plain tobacco region，the field experiment was conducted and choose two varieties Zhongyan 100 and Yuyan No.9 as materials. Results showed that，the leaf SPAD value of two varieties has certain change with the change of light intensity in one day. To comprehensively consider the factors such as stability and maneuverability of measurement，8：00 AM to 10：00 AM is the best determination period. After alcohol-tempon to clean site and test window，the measured results had significant changes compared with before. The leaf SPAD value of two varieties in different sites such as resettling period， vigorous growing period and mature period were analyzed，and it was found that，compared with the other leaves，the determination results variation coefficient of the top fully expanded third leaf was low，so the determination results had better stability；Dividing the top of the third leaf into 10 loci and it was found that the variation coefficient and representation coefficient of mean value of the main central vein left and right sides were low，so the results of the stability and the representative were the best. 　　Key words： SPAD chlorophyll meter； flue-cured tobacco； Henan plain tobacco region 　　植物全氮可以很好地反映植物的氮?I养状况，并且与作物产量也有较好的相关性[1]，因此植物全氮是一个很好的诊断指标。在烤烟整个生育期内，烟株生长和生理代谢都对氮素十分敏感[2]。然而在中国烤烟生产中存在着过量或过迟施氮的问题，这不仅增加生产成本、降低烟叶产质量，还因为肥料利用率较低引起环境污染[3]。河南作为浓香型烟叶最重要的产区，近年来备受品种的困扰，而外引品种并不能充分彰显