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桃小食心虫产卵的寄主选择特性
摘要:为了解桃小食心虫产卵对果实的选择特性,采用固相微萃取(SPME)与气质联用(GC-MS)测定不同苹果挥发性成分。选取易受桃小食心虫危害的金冠苹果具有代表性的挥发物质(乙醛、丁醛、丁醇、己醇、糠醛、庚酸、乙酸乙酯、乙酸丁酯、乙酸戊酯、乙酸己酯、丙酸丙酯)的不同浓度进行桃小食心虫引诱试验。结果表明,0.1和1.0 mg/mL的乙酸乙酯、0.1 mg/mL的乙酸丁酯、1.0 mg/mL的乙酸戊酯、6.0和60.0 mg/mL的乙酸己酯、0.1 mg/mL的丙酸丙酯具有显著的效果;并利用正交试验方法得出几种效果较好的混合配比。再利用人工气候箱进行产卵引诱试验,得出0.1 mg/mL乙酸乙酯、1.0 mg/mL乙酸丁酯、10.0 mg/mL乙酸戊酯、5.0 mg/mL乙酸己酯、0.1 mg/mL丙酸丙酯的混合配比为试验的最佳引诱物质。
关键词:桃小食心虫;寄主选择性;引诱;产卵
中图分类号:S436.621.2文献标识码:A文章编号:0439-8114(2011)11-2236-04
Study on the Host Selection Process of Carposina niponensis Oviposition
WANG Xiao-minga,WANG Hong-pingb,L?? Shu-xiaa,YANG Feng-yia,ZHANG Zhea
(a.College of Biological Science and Technology; b.College of Plant Protection,Shenyang Agricultural University, Shenyang 110866,China)
Abstract: To study the selective characteristics of oviposition of Carposina niponensis Walsingham on fruit, solid phase micro extraction(SPME) and gas chromatography mass spectrum(GC-MS) were applied to analysis the volatiles in apples. The representative volatiles of apples (acetaldehyde, butanal, butylalcohol, hexanol, furfural, heptanoic acid, ethyl acetate, acetic acid-butyl ester, acetic acid-pentyl ester, acetic acid-hexyl ester, propanoic acid-propyl ester) which could be easily damaged by C. niponensis were selected as the attractant to the bugs. And orthogonal experiment was taken to obtain a better mixed ratio to attract the bugs. The results showed that 0.1 mg/mL and 1.0 mg/mL of ethyl acetate, 0.1 mg/mL of acetic acid-butyl ester, 1.0 mg/mL of acetic acid-pentyl ester, 6.0 mg/mL and 60.0 mg/mL of acetic acid-hexyl ester, 0.1 mg/mL of propanoic acid-propyl ester had remarkable effects. An oviposition experiment was taken in the artificial climate box; and that mixed ratio of 0.1 mg/mL ethyl acetate, 1.0 mg/mL acetic acid-butyl ester, 10mg/mL acetic acid-pentyl ester, 5.0 mg/mL acetic acid-hexyl ester, 0.1mg/mL propanoic acid-propyl ester was most attractive.
Key words: Carposina niponensis Wa
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