大鼠慢性脑低灌注模型再灌注脑组织HIF1αVEGF表达意义.docVIP

大鼠慢性脑低灌注模型再灌注脑组织HIF1αVEGF表达意义 　　【摘要】 目的 观察缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）在大鼠慢性脑低灌注后再灌注脑组织中表达的意义。方法 SPF级雄性SD大鼠24只，随机分为两组，假手术组（S组）和再灌注组（R组），每组12只。建立大鼠慢性脑缺血模型，6周后恢复血流灌注，于再灌注前即刻（R0）、再灌注1 h（R1）、24 h（R2）及72 h（R3）行激光多普勒血流探测仪（LDPI），检测脑皮层血流灌注量；其后采用qRT-PCR法，检测HIF-1α-mRNA、VEGF-mRNA在脑组织中的表达。结果 大鼠慢性脑缺血再灌注后1 h，实验侧脑皮层血流灌注量较对侧下降（73.12±26.75）%，再灌注后24 h脑皮层血流灌注量有所恢复，72 h仍未达到基线水平；与S组比较，各组大鼠再灌注后的不同时点HIF-1α-mRNA、VEGF-mRNA均表达增高（P0.05）。结论 HIF-1α、VEGF在大鼠慢性脑低灌注再灌注后表达上调，参与大鼠脑血管增殖及活性增强的病理过程，改善脑血流动力学。 　　【关键词】 再灌注； 脑； 缺氧诱导因子-1α； 血管内皮生长因子； 大鼠 　　Expressions of HIF-1α and VEGF in a rat model of chronic cerebral hypoperfusion and reperfusion JIANG Nan，XIAO Liang-can，RONG Jian. First Affiliated Hospital， Sun Yat-sen University，Guangzhou 510080，China 　　【Abstract】 Objective To observe the expressions of hypoxia-inducible factor-1α （HIF-1α） and vascular endothelial growth factor（VEGF） in a rat model of chronic cerebral hypoperfusion and reperfusion. Methods 24 healthy SD rats were randomly divided into Sham group（group S，n12）and reperfusion group （group R，n12）.The modified chronic cerebral hypoperfusion and reperfusion （CCH） animal model of rat had been established base on the Yassaristudy work. The blood flow perfusion of cerebral pallium was detected by using a Lasser Doppler Perfusion Imager（LDPI） before reperfusion （R0），1h after reperfusion （R1），24h after reperfusion （R2） and 72h after reperfusion （R3）. Rats were sacrificed at R3 after reperfusion,respectively. The expressions of HIF-1α and VEGF gene were inspected at molecular levels with qRT-PCR technique. Results The estimated reduction of the cerebral blood flow perfusion was （73.12±26.75）% at AVF side compared with the values inspected at the opposite side at R1.The cerebral blood flow perfusion recovered partially at R2，but did not reach to the baseline level at R3. Compared with group S，the expressions of HIF-1α and VEGF mRNA in group R after reperfusion were significantly increased （P0.05）. Conclusion The expressions of HIF-1α and VEGF