宫颈病变检测人乳头瘤病毒感染意义.docVIP

宫颈病变检测人乳头瘤病毒感染意义 　　[摘要] 目的 探讨人乳头瘤病毒（HPV）感染与宫颈病变的关系。方法 用荧光定量PCR 法检测15例正常宫颈，57例宫颈尖锐湿疣，78例宫颈上皮内瘤变（CIN），43例宫颈尖锐湿疣合并CIN，13例宫颈癌的 HPV 感染情况。 结果本组宫颈病变中HPV 感染率92.72%，单一型HPV感染率89.52%，高危型HPV 感染率98.25%；宫颈尖锐湿疣以HPV52感染为主（41.18%）；CIN 以HPV16感染为主（41.67%）；尖锐湿疣合并CIN感染HPV58者占25.00%，感染HPV16和HPV52的各占15.00%；60.00%宫颈癌感染HPV16；多重型HPV感染以HPV亚型双重感染为主。 结论 HPV检测对于宫颈病变的筛查及临床分流监测有重要价值。 　　[关键词] 宫颈病变；人乳头状瘤病毒；宫颈上皮内瘤变；宫颈癌；尖锐湿疣 　　[中图分类号] R711.3[文献标识码] B[文章编号] 1673-9701（2014）15-0145-03 　　 　　Significance of infection with human papillomavirus in cervix lesions 　　HAN Shuzhen1 WANG Fuqiang2 WANG Zhijing2 WANG Dujuan1 ZHAO Yinghai2 CHEN Xiaoyi2 　　1.Department of Pathology, Affiliated Dongguan Houjie Hospital, Guangdong Medical College,Dongguan 523945,China;2.Department of Pathology, Basic Medical Sciences of Guangdong Medical College,Zhanjiang 524023,China 　　[Abstract] Objective To investigate the relationship between human papillomavirus infection and cervix lesions. Methods HPV infection was detected by fluorogenic quantitative polymerase chain reaction in all specimens included 15 cases of normal cervix, 57 cases of cervical condyloma acuminatum, 78 cases of cervical intraepithelial neoplasia（CIN）, 43 cases of cervical condyloma acuminatum combining CIN, 13 cases of cervical carcinoma. Results In this groups of cervix lesions,HPV infection rate was 92.72%, HPV single infection rate was 89.52%, high risk HPV subtypes infection rate was 98.25%%. Cervical condyloma acuminatum cases were most commonly infected with HPV52(41.18%). CIN cases were most commonly infected with HPV16(41.67%). Cervical condyloma acuminatum combining CIN cases were more often infected with HPV58 (25.00%), HPV16 (15.00%) and HPV52 (15.00%). 60.00% of cervical carcinoma cases were infected with HPV16. Multiple infection HPV subtype cases were more often infected with dual infection subtype. Conclusion The detection of HPV infection has great value for screening and triage of patients with cervix lesions. 　　[Key words] Cervix l