UCMSCs经枕大池移植治疗发育鼠HIE后遗症实验分析-小儿神经系统疾病专业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 4 4 Part Ⅱ Objective This study was designed to transplant the Mesenchymal Stem Cells derived from human umbilical cord into cisterna magna of the HIE rat model, and to investigate their survival,differentiation in the rat brain.and to have their Behavioral Research. Methods UCMSCs were isolated from human umbilical cord delivered after full-term normal cesarean section, and labeled by DII. Pregnant rats were randomized divided into experimental group and control group.Animal models were built by ligating both sides of the uterine arteries in full-pregnant (20 days) experimental group rats for 15 minutes. The experimental group neonatal rats were divided into stem cells group and PBS group at random. The labeled UCMSCs were injected into cisterna magna of the stem cells group rats, while PBS without UCMSCs was injected into that of PBS group. At 1 and 6 weeks after transplantation, the brain tissues were sliced and examined by immunohistochemical analysis. Record the scores of rope suspension test、 rotarod test, and Morris water maze at different time points. Results 1．ethology: (1)、After transplantation ,the stem cells group rats longer than PBS group rats in rope suspension test.(2)、the Rota-Rod task: three weeks latter, residence time of the stem cells group rats was longer than PBS group rats.(3)、Morris water maze: time for finding hidden platform latency of each rat was gradually reduced; the mean escape latency of model group was significantly longer than control group; the swimming time in the platform quadrant in model group was shorter than control group. The swimming time in the target limited have no different between the stem cells group、PBS group and control group.2.immunohistochemistry：At 1 week after transplantation, DII, nestin, neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) positive cells were found in the hippocampal dentate gyrus、motor cortex of the stem cells group rats. Conclusion Th