TNF-α基因多态性与青海地区藏族人群乙肝病毒易感性的相关性研究-外科学(肝胆胰腺疾病)专业论文.docxVIP

青海大 青海大学医学院 2010 级攻读硕士学位研究生毕业论文 PAGE PAGE 3 Abstract Objective:Analysis of TNF-α gene region in Qinghai Tibetan crowd two site s - 238-308 and the relationship between the susceptibility to chronic hepatitis B, and screen out main related sites, for hepatitis B areas in Qinghai Tibet- an populations vulnerable to risk the molecular prediction lay the foundation. Thus for clinical diagnosis and prevention of chronic hepatitis B to provide n- ew ideas and methods. Methods:(1) The object of study: In 250 cases of chronic hepatitis B patients tibetans in Qinghai area (case group) and 134 cases of healthy subjects (contro l group) as the research object. Gene polymorphism analysis: using blood genomic DNA extraction kit extra cted from peripheral blood, small use of flight mass spectrometry determination of TNF alpha gene locus, - 238 A /A、-238 A/G、- 238 G/G，-308 G/A、 308 G/G genotype. Data analysis: calculate each genotype and allele frequency, comparison of each genotype differences between groups, and Hardy-Weinberg equilibrium ali gnment inspection.Applying logistic multivariate regression analysis the correlati on of susceptible genotypes with hepatitis B. Results -238 in various clinical types of distribution: -238-A/G in chronic viral hepatitis B and the control group were 8.4% and 8.2% respectively, the statisti cal analysis results show that the distribution frequency of no significant differ ence between groups; 238A/G in chronic viral hepatitis B and control group respectively 0.4% and 0.0%, after statistics analysis results show that the distribution frequency of no significant difference between groups; 238G/G in chronic viral hepatitis B and the control group were 91.2% and 91.8% respectively, the statistical analysis results show that the distribution freq uency of no significant difference between groups; -308 in various clinical types of distribution: -308-G/A in chronic viral hep atitis B