U251在干细胞培养条件下的侧群细胞表型及相关干细胞特性研究-神经外科专业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 5 5 2、There were no CD133(+) cells in both the adherent cells in serum-containing media and the neurosphere cultured in serum-free media; 3、There were 8.17±3.74 Nestin (+) cells per high magnification field in the U251 adherent cells under serum-containing media,but the percentage rose to 37.39±6.31 when transfering the cells to serum-free media（P0.05）; 4、Comparing the self-renewal capacity of the adherent cells and the neurospheres, there was significant difference using limiting dilution assay（P0.05）; 5、The tumor-forming ratio in U251 adherent cells group was 6/15, while the ratio was 12/15 in tumor neurospheres implanting group; and the occurrence of neurological symptoms in tumor spheres implanting group was earlier than the adherent cells group. Conclusions: 1、The proportion of the SP cells in U251 adherent cells under serum-containing media was extremely low,but when cultured for three months in serum-free media ,the propotion increased significantly; 2、Together with the increase of the SP propotion, other “stemness” characteristics such as nestin expression, self-renewal capacity , and the tumorigenicity in vivo were enriched correspondingly; 3、The changes of the “stemness” characteristics in stem cells abovementioned are independent of the expression of CD133; 4、It was unclear whether the increased propotion of SP cells and corresponding “stemness” characteristics were due to the enrichment of originally existing GSC in the U251 cell line, or due to a retro-differentiation process occuring in vitro condition, which remains to be answered. Key Word: U251 glioma cell line glioma stem cells tumor neurosphere side population cells 1 1 主要缩略词表 缩略词 英文全称 中文全称 FACS flow automatic cytometry separation 流式细胞仪 SP side population cells 侧群细胞 BTSC brain tumor stem cells 脑肿瘤干细胞 GSC glioma stem cells 胶质瘤干细胞 NSC neural stem cells 神经干细胞 ES embryonic stem cells 胚胎干细胞 NOD-SCID non-obese diabetic/svere combined immunodeficiency disease 非肥胖型糖