TL1A在ox-LDL诱导HUVECs凋亡中的作用-病理学与病理生理学专业论文.docxVIP

PAGE PAGE 10 凋亡率随着抗体浓度的增加从(17.67±2.69)%下降到(5.01±1.44)% (P 0.05)， Caspase-3 的活性也随着 anti-TL1A Ab 浓度的增高而下降。 结论 1. ox-LDL 可诱导体外培养的人血管内皮细胞 TL1A 高表达 2. TL1A 在 ox-LDL 诱导人血管内皮细胞凋亡起着重要作用 关键词 氧化低密度脂蛋白 人脐静脉内皮细胞 凋亡 肿瘤坏死因子配体相关 分子 1A Effects of TL1A on the ox-LDL Induced Apoptosis in Vascular Endothelial Cells Abstract It is widely appreciated that oxidatively modified form LDL (ox-LDL) is a critical factor in atherogenesis. Endothelial dysfunction elicited by ox-LDL has been identified in the course of atherogenesis and its complications and the pathogenesis has not yet been elucidated. In recent years, investigations found that TNF-like ligand 1 aberrance (TL1A), as the important and specific endogenous vascular endothelial growth inhibitor, could be secreted by the endothelial cells and induce apoptosis of endothelial cells. TL1A, belonging to tumor necrosis factor ligand superfamily (TNFS), the ligand of death receptor3 (DR3) and decoy receptor (DcR3), has been identified and coloned. Inducing apoptosis in vascular endothelial cells is one of the form of endothelial dysfunction, playing an important role in the development of atherogenesis. We investigate the expression of TL1A mRNA and protein induced by ox-LDL and the effect of TL1A under the condition of ox-LDL-induced cell apoptosis. Objective This study is targeted to observe the expression of TL1A in human umbilical vein endothelial cell (HUVECs) under the ox-LDL condition and to investigate the effects of TL1A on the ox-LDL induced apoptosis in vascular endothelial cells. Methods HUVEC were treated with different concentrations of ox-LDL (0,20,40,60,80μg/ml) for 24 hours or 40μg/ml ox-LDL for different time (0,6,12,24,48h) in vitro. The expression of TL1A was evaluated by reverse transcriptase PCR (RT-PCR) and western blotting. Adding different concentrations of TL1A monoclonal antibody (1,3,9 μg/ml) to HUVECs which were cultured in 40μg/ml ox-LDL or recombinant human TL1A 50ng/mL or 40μg/ml ox-LDL to HUVEC whic