法国蓝薰衣草挥发油改善记忆障碍药效评价研究.docVIP

法国蓝薰衣草挥发油改善记忆障碍药效评价研究 　　[摘要] 为研究薰衣草挥发油改善记忆障碍的潜在应用价值，采用GC-MS技术分析了薰衣草挥发油的化学组成，并建立小鼠阿尔茨海默病（Alzheimer′s disease， AD）模型，以跳台实验和脑组织中T-SOD， GSH-PX， CAT等抗氧化酶活性及其产物MDA含量的变化等研究了薰衣草挥发油的改善记忆障碍功能。实验结果表明，GC-MS技术分析鉴定了薰衣草挥发油55种化学成分，主要为萜烯、萜醇和酯类化合物，樟醇和乙酸芳樟酯含量最高，占总挥发油的49.71%。薰衣草挥发油给药组均显著改善了小鼠跳台记忆能力，对小鼠脑组织中GSH-PX、CAT和T-SOD水平均显著高于模型组（P0.05），MDA在模型组中达到最高，药物组MDA则与正常组水平无差异，体现了薰衣草挥发油显著的抗脑组织氧化活性，存在诱导神经元氧化应激减少的可能性，使记忆获得性障碍出现扭转的作用。 　　[关键词] 薰衣草挥发油； 记忆障碍； 成分分析； 抗氧化酶 　　[Abstract] In order to study the potential application value of lavender volatile oil （LVO）， the chemical composition of the volatile oil of lavender was analyzed by GC-MS， and the mouse model of Alzheimers disease （AD） was established. Additionally， the antioxidant enzymes activity of T-SOD， GSH-PX， CAT and MDA content were studied. Experimental results showed that 55 kinds of chemical constituents including terpene， terpene alcohol and ester compounds from LVO were identified， and the content of linalool and linalyl acetate was the highest， accounting for 49.71% of the total volatile oil. The ability of mouse platform memory was improved significantly. The levels of GSH-PX， CAT and T-SOD of mouse brain tissue in the treatment group were significantly higher than those in the model group （P0.05）. The level of MDA reached the maximum value in the model group， while there was no notable difference between the levels of MDA in the drug group and the normal group. The result indicated the significant oxidative activity of LVO， the possibility of induced oxidative stress reduction in neurons， and the reversal effect of memory acquired disorder. 　　[Key word] lavender volatile oil； memory impairment； chemical composition； antioxidant enzymes activity 　　挥发油，一类来源于植物花、茎、叶或根等部位，经水蒸气蒸馏、压榨法或CO2超临界萃取等方法提取的具有一定香味的挥发性油状物质，其组成成分复杂多样，主要含各种萜类、芳香族、脂肪族和含氮含硫化合物等。植物挥发油，广泛分布于中药材中，如在心脑血管改善中应用的川穹、麝香、牛?S、花椒和石菖蒲等挥发油，在呼吸系统中止咳、平喘、祛痰消炎等作用发挥的陈皮、姜黄、枇杷、艾叶、麻黄等挥发油，以及改善胃肠道系统的枳实、高良姜等挥发油，在医学领域已得到普遍关注[1]。国内外对挥发油在抗抑郁、缓解焦虑、抗氧化、抑菌消炎以及抗肿瘤等方面的生物活性研究十分活跃[2-3]。 　　薰衣草，芳香疗