牛白藤叶中绣球酚的分离及含量测定.docVIP

牛白藤叶中绣球酚的分离及含量测定 　　摘要：目的 对牛白藤叶中的绣球酚进行分离鉴定并建立其含量测定方法，为牛白藤药材的进一步开发研究提供参考，同时对不同产地、不同批次牛白藤叶中绣球酚的含量进行比较。方法 采用HPLC，使用Zorbax Eclipse XDB-C18色谱柱（150 mm×4.6 mm，5 μm），流动相为乙腈∶0.02 mol/L磷酸二氢铵（用磷酸调节pH值至3.0）=33∶67，流速1.0 mL/min，柱温35 ℃，检测波长350 nm。结果 绣球酚在0.412～10.300 μg范围内线性关系良好（r=1.000 0）；平均回收率为99.09%（RSD=0.53%）；精密度及重复性均良好，RSD均小于1%。不同产地及批次牛白藤叶中绣球酚含量为0.03%～0.38%，其中广西产含量最高。结论 本研究建立的方法操作简便、准确，专属性强，重复性好，可用于牛白藤叶中绣球酚的含量测定；不同产地、不同批次牛白藤叶中绣球酚含量差异较大，其中广西、广东产牛白藤中绣球酚含量较高。 　　关键词：牛白藤；绣球酚；含量测定 　　DOI：10.3969/j.issn.1005-5304.2018.03.021 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2018）03-0094-04 　　Abstract： Objective To conduct isolation identification of hydrangenol and establish a method for content determination of hydrangenol in the leaves of Hedyotis hedyotidea （DC） Merr； To provide references for further development and research of Hedyotis hedyotidea； To compare the contents of hydrangenol in the leaves of Hedyotis hedyotidea of different production areas and different batches. Methods Hydrangenol showed a good linear relationship within the range of 0.412?C10.300 μg （r=1.000 0）， with the average recovery of 99.09% （RSD=0.53%）. Good precision and repeatability were achieved with the RSDs smaller than 1.0%. The content range was 0.03%?C0.38% for hydrangenol in the leaves of Hedyotis hedyotidea of different production areas and different batches， and the highest contents appeared in those from Guangxi. Conclusion The method is simple， accurate， specific， reproducible and can be used for the content determination of hydrangenol in the leaves of Hedyotis hedyotidea； the contents of hydrangenol in the leaves of Hedyotis hedyotidea of different production areas and different batches are different， and contents in samples from Guangxi and Guangdong are higher. 　　Keywords： Hedyotis hedyotidea （DC） Merr； hydrangenol； content determination 　　牛白藤为茜草科耳草属植物牛白藤Hedyotis hedyotidea（DC）Merr的干燥藤茎，又名牛奶藤、土加藤、甜茶，为两广地区民间习用中草药。其味微甘，性凉，归脾、肝经，功效清热解毒、祛风活络、消肿止痛，用于感冒发热、肢体筋骨酸痛、风湿痹痛、跌打损伤。叶可清热解暑，用于感冒风热咳嗽、湿热泄泻[1]。 　　近年