不同浓度的一氧化氮对冻干移植骨修复骨缺损影响的实验研究-外科学专业论文.docxVIP

中文摘要 嚣的:比较深低婆豆冷冻干燥骨和豆豆硝基化牛血清蛋白的复合物与单独应用深低温 冷吕京平:嗓音在修复大段骨缺损中的效果，探讨抖。在异体组织移植中的作思， 方法:采用新西兰大耳白兔挠骨中段切除 l.5cm 骨干和管膜的实验动物模型，同 时锻备能在体内持续缓慢释放…载化氮的变穰基化卒觅靖蛋自?将 48 只兔髓机分成 3 恕。 A 怨:低浓度亚硝3恶化牛血清蛋白与深低滋冷涛、干燥骨的复合物〈低浓度 NO 冻 子骨组); B 组:高浓度亚硝基化牛血清蛋白与深低温冷冻干燥骨的复合物〈商浓度 NO 涛、于管组); C 运=深{瓷器豆冷冻干燥、营〈单纯冻子?等级 λ 分别在术后 3 6、争及 12 周对上述三锐的骨缺损模型进行 X 绒片、组织学检菇及钙含量测定非并在 12 周处 死动物韵，测量是动物体内 NO 的含量。 . - 结果: (1)低浓度 NO东子管经修复督缺损的效果明显好子单纯;朱子营经 (p0.05) 和高浓度 NO 冻干骨组 (PO.Ol)，术应第 12 周缺损区完全性修复，骨髓腔辩:通;单 纯冻平常经穆策管接祭的效果统子高浓度 NO 冻干晋级 (p 吨，部〉台。)12 湾时高浓度 NO 冻子骨组的 NO 含量去离子单纯冻干贷银 CP 0.01)和低浓度 NO 冻二F管组(PO‘05)t 低浓度 N01朱子膏组的 NO 含蠢高于单纯冻千管组 (P0.05)公 结论E 在修复营缺损的过程中， í延浓度 NO 能绽进管愈合，高离浓度 NO 郎对音 愈合过程起到抑制作用帚 关键词=…氧化氮:骨被损:管惨复 E Abstract Objective: To evaluate 郎 effect of di的reot concentration Nitric . Oxíde(NO) 00 repairing of large segenlental bone defec:稳、 Methods: Segemental bone defects for 15mm were created ?n the 48 New Zealand rabb?ts on mid part of the right radi邸，and Nittoso-bovine s臼um albuIDin was made as nìtric oxide donor.48 rabbits were divided into 也ree groups，the defects beìng treated with three kinds of implants ，A:low concentration nitroso-bovìne serum albumin combìned with 会在叹号-dried bone allograftJ B: hìgh concentration nitroso-bovine serum albumin combined with freeze-dried bone allograft ，C: single free-dried bone allograft as control.Radiographic and histologic examination .was made and the amount of calcium in 白.e defect area was tested à:t 3)6，9 and 12weeks after operation. 12 w号:eks before killing 也εrabbits t挝 ωntent of nitrite in the rabbits body w;ωmeasured. Results: The resu1ts show Oroup A was better than Group B (P0.(1) and Group C (P 0.05) in the process ofbone he必吨. and Group C was be柱er than Group B(P 0β5).12 weeks after surgery，mean serum nitrite levels in Group B were higher than in Group A (P 啡.05) and in G玲在p C(P 0.01)应e levels in Group A \\ere higher than in the Gro叩C (P O.05).By 12 weeks，the defects in Group A were repaired completely and the u1edull缸y canal was recanalized. Conc