不同疗程rhEPO对宫内炎症致新生大鼠脑损伤MOG及EPOR表达的影响-儿科学专业论文.docxVIP

万方数据 万方数据 4.rhEPO 促进 MOG 基因及蛋白表达上调的同时伴有 EPOR 蛋白水平升高，提示EPOR 在 rhEPO 在发挥神经修复保护作用机制中发挥作用。 5.rhEPO 治疗宫内炎症致脑损伤应早期治疗，且不同疗程的rhEPO 对髓鞘基因及 蛋白的表达影响不完全相同，14 天的疗程目前为最佳疗程。 硕士研究生 罗刚 （儿科学专业） 指导教师 姜 红 教授 关键词：宫内炎症；早产；脑损伤；促红细胞生成素；髓鞘少突胶质细胞糖蛋白 Effect of Different Erythropoietin Treatment Courses on MOG and EPOR Level in Rats Brain with Intrauterine Inflammation Abstract Objective: To Study the neuroprotective effect of rhEPO on premature rats with intrauterine infection WMD through detection of the changes in MOG and EPOR in brain tissue and assessment of neuroethology. Method: Selected 12 wistar rats that had been pregnant for 15d and divided them into infection group (n=9) and control group (n=3) by ratio 3:1 at random, conducted intraperitoneal injection of LPS (0.3mg/Kg) and equivalent stroke -physiological saline solution on 15th day’s pregnancy of Wistar rats in both groups respectively and conducted cesarean section to take out fetal rats on 21th day’s pregnancy; observed pathological changes by taking out fetal rats (n=6) and new rat brain tissue (n=10) hematoxylin eosin (HE) staining in two groups respectively; randomly selected 32 newborn rats in the control group as the black control group; selected 64 newborn rats in the infection group and randomly divided into EPO treatment group (n=32) and infection control group (n=32). Regarding EPO treatment group, conducted intraperitoneal injection of rhEPO (5000IU/Kg) promptly every day after the birth. Regarding black control group and infection control group, conducted intraperitoneal injection of equivalent saline solution simultaneously. As for newborn rats in three groups, took 8 premature rats on 0h,3rd,7rd and 14th day of their birth respectively and took out brain by perfusing formaldehyde; detected the level of MOGmRNA by RT -PCR,detected the level of MOG prot ein by western blot and EPOR in tissue through elisa; as for these three groups. Result: 1. The mortality of pregnant rats after injectio