康复新液对TNBS诱导大鼠溃疡性结肠炎治疗作用研究.docVIP

康复新液对TNBS诱导大鼠溃疡性结肠炎治疗作用研究 　　【摘要】目的：观察康复新液对2，4，6-三硝基苯磺酸（TNBS）诱导的溃疡性结肠炎模型大鼠的治疗作用。方法：将60只SD大鼠随机分为空白对照组（等体积蒸馏水）、模型对照组（等体积蒸馏水）、柳氮磺吡啶组（SASP，06g/kg）、康复新液高、中、低剂量组（20、10、5mL/kg），每组10只，雌雄各半。采用TNBS灌肠造模，造模后第3天开始灌胃给药，连续12d，给药结束后各组取材进行HE染色，并用ELISA法测定结肠组织中髓过氧化物酶（MPO）、肿瘤坏死因子α（TNF-α）、白介素-1β（IL-1β）和白介素-10（IL-10）的水平。结果：造模后大鼠结肠呈明显溃疡样损伤；与空白对照组相比较，模型对照组大鼠结肠组织中促炎相关因子MPO、TNF-α和IL-1β的含量升高（P005），抗炎因子IL-10含量降低（P005）；与模型对照组比较，康复新液高剂量组大鼠结肠组织损伤减轻，高、中、低剂量组均能降低大鼠结肠组织中MPO、TNF-α含量（P005），也有降低IL-1β含量和升高IL-10含量的作用趋势。结论：康复新液对TNBS诱导的大鼠结肠炎具有良好的治疗效果，机制可能与调节抗炎因子和促炎因子之间的平衡作用有关。 　　【关键词】康复新液；2，4，6-三硝基苯磺酸；溃疡性结肠炎；抗炎作用 　　【中图分类号】R2855【文献标志码】 A【文章编号】1007-8517（2016）21-0010-05 　　Experiment Study of Treatment Effects of Kangfuxin Solution on TNBS-Induced Ulcerative Colitis 　　HU Tingting1RAO Chaolong1GENG Fu neng2DENG Qingxiu1XIE Xiaofang1* 　　1Chengdu University of Traditional Chinese Medicine， Cheng du， 611137， China；2Good doctor pharmaceutical group， Xichang， 615000，China 　　Abstract：Objective To observe the therapeutic effects of Kangfuxin solution on rat model of ulcerative colitis induced by TNBS. Method sixty SD rats were randomly divided into six groups： nomal control groups， model groups， SASP groups， high-dose Kangfuxin groups， medium-dose Kangfuxin groups and low-dose Kangfuxin groups.Each groups have 10 rats including 5 male rats and 5 female rats.TNBS was instilled into the colon of rats to induce ulcerative colitis. 3 days after modeling， every groups were given relevant drugs once a day for 12d. On the day 12，after administration， the rats were sacrificed to collect colon specimens for HE staining. Elisa was performed to detect the levels of MPO， TNF-α， IL-1β andIL-10 in colon specimens of rat. Result Compared with nomal control groups， the contents of anti-inflammatory factors MPO， TNF-α， IL-1β in colon specimens of rats were significantly higher（P005）， pro-inflammatory factor IL-10 was significantly reduced （P005）. Kangfuxin solution can reduce the contents of MPO and TNF-α in colon specimens of