干扰素调节因子8在骨髓增生异常综合征伴原始细胞增多亚型中表达研究.docVIP

干扰素调节因子8在骨髓增生异常综合征伴原始细胞增多亚型中表达研究 　　[摘要] 目的 研究干扰素调节因子8（interferon regulatory factor 8，IRF-8）在骨髓增生异常综合征伴原始细胞增多（Myelodysplastic Syndromes with Excess Blasts，MDS-EB）亚型中是否存在表达异常，并分析其与MDS-EB的相?P性。方法 收集三组共30例实验标本。其中20例为MDS-EB病例标本，分为MDS-EB1组和MDS-EB2组。10例为健康体检的健康对照组。30例标本均采集自受试者外周静脉血，采集后立即进行外周血总RNA提取，并经逆转录PCR后，以实时荧光定量PCR检测各标本的IRF-8 mRNA表达水平。各组平均表达量以RQ值（x±s）表示。 结果 三组样本实时荧光定量PCR的平均RQ值：MDS-EB1组（0.59±0.08），MDS-EB2组（0.26±0.10），健康对照组（1.20±0.26），三组表达水平存在显著差异（P0.01）。MDS-EB组的IRF-8表达水平均显著低于健康对照组（P0.01），MDS-EB2组内IRF-8表达水平低于MDS-EB1组（P0.05）。 结论 IRF-8在MDS-EB中表达水平降低，表明IRF-8在MDS-EB中表达受抑，组间统计结果提示IRF-8的表达可能与疾病的恶性程度及进展相关。 　　[关键词] 干扰素调节因子8；骨髓增生异常综合征伴原始细胞增多；外周血RNA；逆转录PCR；实时荧光定量PCR 　　[中图分类号] R551.3 [文献标识码] A [文章编号] 1673-9701（2017）09-0043-05 　　Study on expression of interferon regulatory factor 8 in myelodysplastic syndromes with excess blasts subtype 　　XU Xudong1 XIAO Xiangyang1 CHEN Long1 WANG Jianchao2 　　1.Zhejiang Chinese Medicine University Second Clinical Medical College， Hangzhou 310053， China； 2.The Second Affiliated Hospital of Zhejiang Chinese Medical University， Hangzhou 310005， China 　　[Abstract] Objective To investigate whether the expression of interferon regulatory factor 8（IRF-8） is abnormal in myelodysplastic syndromes with excess blasts （MDS-EB） subtype and to analyze its correlation with MDS-EB. Methods A total of 30 experimental specimens from three groups were collected. 20 cases were MDS-EB case specimens and divided into MDS-EB1 group and MDS-EB2 group. 10 cases of healthy physical examination were as healthy control group. All the samples were collected from the peripheral venous blood of the subjects. The total RNA was extracted from the peripheral blood immediately after the collection. The expression level of IRF-8 mRNA was detected by real-time fluorescence quantitative PCR after reverse transcription PCR. The average expression of each group was expressed as RQ（x±s）. Results The mean RQ values of real-time fluorescent quantitative PCR in the three groups were M