车前子多糖 地黄多糖对骨髓干细胞增殖的分析-analysis of plantago seed polysaccharide rehmannia polysaccharide on proliferation of bone marrow stem cells.docxVIP

成都中医药大学学位论文 成都中医药大学学位论文 PAGE PAGE 1 摘要 目的：本文以 SD 大鼠骨髓间充质干细胞为研究对象，观察地黄多糖， 车前子多糖对骨髓间充质干细胞增殖的作用。验证药性甘寒，归属于 肝肾经的药物对骨髓干细胞的是否具有相同作用。 方法：取大鼠胫骨和股骨的骨髓进行体外培养并纯化，分别加入多种 浓度药物多糖,二甲基亚矾(DMSO)。然后于倒置显微镜下观察细胞形态 和增殖情况。MTT 比较不同浓度药物多糖对细胞增殖影响。 结果：不同浓度的地黄多糖,车前子多糖都对骨髓间充质干细胞增殖 起到抑制作用,不同浓度组之间随药物浓度减小对细胞抑制减小。二甲 基亚矾(DMSO)作为公认的促进骨髓间充质干细胞分化的药物,对骨髓 间充质干细胞增殖的抑制作用高于药物多糖。 结论：通过显微镜下观察,地黄多糖、车前子多糖在体外细胞培养试 验中对骨髓间充质干细胞分化可能有诱导作用。但是地黄多糖，车前 子多糖对骨髓间充质干细胞增殖却是起到了抑制作用。 关键词：骨髓干细胞；细胞增殖；地黄多糖；车前子多糖 Abstract Objective: to SD rat bone marrow mesenchymal stem cells for research, observation Dihuang polysaccharide, Plantago polysaccharide on bone marrow mesenchymal stem cell proliferation.gan ， hanauthentication properties, attributed to liver and kidney by the drug on bone marrow stem cells have the same effect whether. Methods: Rat tibia bone and femur bone marrow culture in vitro and purified by adding a wide range of concentrations of drugs were polysaccharides, Fanshan DMSO (DMSO). And then observed under inverted microscope on cell morphology and proliferation. MTT comparison of different concentrations of the drug effects of polysaccharides on cell proliferation. Results: Different concentrations of Rehmannia polysaccharide, Plantago polysaccharide of bone marrow mesenchymal stem cells play an inhibitory effect of different concentrations of the drug concentration between the groups decreased with the suppression of the cell decreases. Fanshan DMSO (DMSO) as recognized by the promotion of bone marrow -derived mesenchymal stem cells of drugs, of bone marrow mesenchymal stem cells than the drug polysaccharide. Conclusion: The observation under the microscope, Rehmannia polysaccharide, Plantago polysaccharide in vitro cell culture experiments on bone marrow-derived mesenchymal stem cells may be induced by the role. But Dihuang polysaccharide, Plantago polysac charide on bone marrow-derived mesen chymal stem cells