周期张应力下tdscs与plla-cl-col支架构建组织工程肌腱的研究-construction of tissue engineering tendon with tds cs and plla - cl - col scaffold under periodic tensile stress.docx
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周期张应力下tdscs与plla-cl-col支架构建组织工程肌腱的研究-construction of tissue engineering tendon with tds cs and plla - cl - col scaffold under periodic tensile stress
万方数据
万方数据
全文结论 88
参考文献 89
文献综述 肌腱干细胞的研究进展 99
参考文献 108
在攻读博士学位期间取得的成果 112
致 谢 114
第三军医大学博士学位论文
第三军医大学博士学位论文
缩略词表
缩写
3D
英文名称
three-dimensional
中文名称
三维
ECM
extracellular matrix
胞外基质
P(LLA-CL)
poly(l-lactide-co-ecaprolactone)
聚乳酸-聚己内酯
TCs
tendon cells
肌腱细胞
nm
nanometer
纳米
μm
mcrometer
微米
mm
millimeter
毫米
cm
centimeter
厘米
Mw
molecular weight
分子量
Co. Ltd.
Company Limited
有限公司
HFIP
1,1,1,3,3,3-hexafluoro-2-propanol
六氟异丙醇
w/v
Weight in Volume
重量/体积百分比
mL
milliliter
毫升
h
hour
小时
kV
kilovolt
千伏
r/min
revolutions per minute
转/分
SEM
scanning electron microscopy
扫描电子显微镜
V
volume
体积
FTIR
Fourier transform infrared spectroscopy
傅里叶变换红外光谱
Kg
kilogram
千克
mg
milligram
毫克
ug
microgram
微克
DMEM
Dulbecco’s modified Eagle’s medium
DMEM 培养基
TCPSs
tissue-culture polystyrene plates
组织培养皿
PBS
Phosphate buffered saline
磷酸盐缓冲溶液
min
minute
分钟
CCK-8
Cell Counting Kit-8
CCK-8 细胞计数试剂盒
HE
Hematoxylin-eosin staining
苏木素-伊红染色
1
DAPI
4,6-diamidino-2-phenylindole hydrochloride
4,6-二脒基-2-苯基吲哚
LSCM
Laser scanning confocal microscopy
激光共聚焦
PCR
polymerase chain reaction
聚合酶链反应
cDNA
complementary deoxyribonucleic acid
互补脱氧核糖核酸
MPa
Megapascal
兆帕
ID
Identity
标识
SD
standard deviation
标准差
GPa
Gigabit pascal
千兆帕
TDSCs
Tendon derived stem cells
肌腱干细胞
Col
Collagen
胶原
MSCs
mesenchymal stem cells
间充质干细胞
mRNA
message RNA
信使核糖核酸
dsDNA
double-stranded deoxyribonucleic acid
双股脱氧核糖核酸
Runx2
Runt-related transcription factor 2
成骨特异性转录因子 2
PMSF
Phenylmethanesulfonyl fluoride
苯甲基磺酰氟
CM-Dil
chloromethyl-benzamidodialkylcarbocyanine
氯甲基苯甲酰氨
IVIS
Interactive Video Information System
交互式视频信息系统
HRP
Horseradish peroxidase
辣根过氧化物酶
DM
dynamic group
应力刺激组
NM
static group
静置组
FBS
Fetal bovine serum
胎牛血清
pH
power of hydrogen
酸碱度
OD
Optical density
光密度
DAB
diaminobenzidine
3,3-二氨基联苯胺
FITC
fluorescein isothiocyanate
异硫氰酸荧光素
EDTA
ethylene diamine tetracetic acid
乙二胺四乙酸
KD
Kilo-dillton
千道尔顿
2
Study of cyclic tensile stress stimulation on the maturation of TDSCs-P(LLA-CL)-Col scaffold constructs for tendon tissue engineering
Abstrac
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