初步探讨肿瘤转移抑制基因tmsg-1入核机制及检测其在人前列腺癌中表达-to investigate the mechanism of tumor metastasis suppressor gene tmsg - 1 entering the nucleus and to detect its expression in human prostate cancer.docxVIP

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初步探讨肿瘤转移抑制基因tmsg-1入核机制及检测其在人前列腺癌中表达-to investigate the mechanism of tumor metastasis suppressor gene tmsg - 1 entering the nucleus and to detect its expression in human prostate cancer.docx

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初步探讨肿瘤转移抑制基因tmsg-1入核机制及检测其在人前列腺癌中表达-to investigate the mechanism of tumor metastasis suppressor gene tmsg - 1 entering the nucleus and to detect its expression in human prostate cancer

Preliminary study on the nuclear import mechanisms of tumor metastasis suppressor gene 1 Abstract Objective Tumor metastasis suppressor gene 1(TMSG-1/LASS2) is an important metastasis suppressor gene which is newly discovered. In normal and stimulated conditions, we detected the expression of TMSG-1 and apoptosis of Hela cells, and explored the nuclear import mechanism of TMSG-1 protein. Methods The expression of TMSG-1 was detected by immunofluorescence before and after H2O2 stimulation. After stimulation of different doses of H2O2, reverse transcription PCR and Western blot was used respectively to measure the expression of TMSG-1 mRNA and protein. Then we detected the different incidence of apoptosis by Flow Cytometry after H2O2 stimulation. Results Immunofluorescence results showed that the TMSG-1 expression in Hela cells could be detected both in natural state and stimulated state. The expression was much higher in stimulated state than in natural state. Reverse transcription PCR results showed that the TMSG-1 expression in Hela cells in stimulated state was much higher than that in natural state. With the increasing doses of H2O2, the TMSG-1 expression increased gradually. The obvious group was the H2O2 dose of 1.5mM. Western blot results also showed that the TMSG-1 expression in cells in stimulated state was significantly higher than that of natural state(P0.05). The most obvious group was the H2O2 dose of 1.5mM. Flow cytometry results showed that there were different incidence of apoptosis before and after H2O2 stimulation(P0.05), and the most obvious group was the H2O2 dose of 1.5mM. Conclusion The TMSG-1 expression was significantly higher in cells of H2O2 stimulation than cells in natural state. After stimulation of quantitative doses of H2O2, TMSG-1 expression levels all increased, so as the cell number of apoptosis. Therefore, we conjectured that TMSG-1 nuclear import could be promoted by H2O2 stimulation, then TMSG-1 protein would promote cell apoptos