青杞1号组织培养关键技术分析-analysis on key techniques of tissue culture of qingqi no.1.docxVIP

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青杞1号组织培养关键技术分析-analysis on key techniques of tissue culture of qingqi no.1.docx

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青杞1号组织培养关键技术分析-analysis on key techniques of tissue culture of qingqi no.1

Abstract Abstract II II Abstract In this study, Lycium barbarum var qingqi1was treated as the research object.Different explant disposal, blade callus induction, callus multiplication,callus differentiation, stem adventitious bud induction and rooting culture were setted as 6 periods.The growth status in every period was studied to analyse and summarize the best treatment efficiency, and explore the best hormone combination.The result showed that:the best selecting materials time isMay and June.May and Juneare the best time for sampling. At this period,the young sprouts growed exuberantly, and the toxic substance accumulated less; The best concentration of HgCl2 is 0.10mg/L,the best suitable time of disinfection is 10min.It could kill the bacteria and would not hurt the explants.The best hormone combination of leaf blade callus induction is 6-BA0.7mg/L+NAA0.7mg/L.They had highly altitudinal redifferentiation ability and exuberant multiplication ability. The best hormone combination of callus multiplication is 6-BA1.0mg/L+IBA0.8 mg/L +NAA 0.2mg/L.It reached the propagation fastigium in the 15th. Callus volume increased about 5cm3. They have higher differentiation ability. The best hormone combination of callus differentiation is KT1.3mg/L+NAA0.02mg/L, the leaf primordium began to appear in the 6th, a lot of leaf primordiums appeared in the 9th,the differentiation rate was 86.7% and the buds were exuberant. The best hormone combination of adventitious buds induction is 6-BA0.8mg/L+NAA0.05mg/L,the induction rate was 86.7%, the length of average bud was 5.83cm, and the new buds was exuberant.The best hormone combination of rooting culture is IBA0.3mg/L+NAA0.3mg/L,it was one week that the root began to appear,the rooting rate was 88.3%, the root and bud were exuberant. Key words: Lycium barbarum var qingqi1 Explant Callus Induction Multiplication Differentiation Rooting 青海大学硕士学位论文目录青海大学硕士学位论文目录 PAGE PAGE IV 目录 HYPERLINK \l _bookmark0 引言 1 第一