改良法制作兔VX2骨肿瘤模型实验研究.docVIP

改良法制作兔VX2骨肿瘤模型实验研究 　　摘要：目的 通过比较改良法与传统法制作兔VX2骨肿瘤模型，明确改良法可明显改善肿瘤细胞经骨孔外渗情况。方法 取传代VX2肿瘤荷瘤兔，将肿瘤制备成肿瘤组织块，分别植入22只成年家兔双侧胫骨内制成兔VX2骨肿瘤模型。所有家兔左侧胫骨采用改良法植入肿瘤组织块，即通过套管植入肿瘤组织并给予骨蜡封堵；而所有右侧胫骨直接经穿刺骨孔植入肿瘤组织块。模型制作3w后，给予所有家兔患肢行彩色超声检查，明确有无软组织包块形成；同时处死家兔，取骨孔周围软组织行病理检查，明确有无肿瘤细胞转移。结果 造模3w后，双侧胫骨成瘤率100%。超声显示左侧组发现软组织包块2例，右侧组6例；病理检查示左侧组肿瘤细胞经骨孔外渗率（23%，5/22小于右侧组（55%，12/22）。将两组穿刺骨孔肿瘤细胞外渗率进行比较，差异有统计学意义（χ2=4.69，P0.05）。结论 改良法制作骨肿瘤模型可改善肿瘤细胞经骨孔外渗情况，是一种较为理想的造模方式。 　　关键词：VX2肿瘤；骨肿瘤；模型；病理学 　　Study on the Improved Method in Establishment of VX2 Bone Tumor Model in Rabbit 　　CAO Hai-ying，FENG Zhen，WEI Jun-qiang，ZHAO Jing-xin，YU Tao，JIN Yu 　　（Chengde Medical College， Chengde 067000，Hebei，China） 　　Abstract：Objective To investigate the efficacy between the traditional method and the improved method in establishment of VX2 bone tumor model in rabbit in order to prove the improved method can improve tumor cells spreading. Methods After separating the VX2 tumor from cancer bearing VX2 rabbit， the tumor tissue blocks were prepared. the tissue blocks were embedded into the right tibias 22 rabbits through the puncture hole ，and all the puncture holes were closed by bone wax. Color Doppler Ultrasound was used to assess the soft enclosed mass near the puncture hole after 2 weeks. The pathological study also was carried out for the soft tissue near the holes after 14 days. Results Only 2 cases from the left group showed the soft mass around the puncture holes after 14 days， but there were 6 cases around the puncture holes of all the 22 cases of the right group. The pathological showed there were 5 cases（23%，5/22） that we can find the tumor cells in the soft tissue around the puncture hole in the left group， but 12 cases（55%，12/22）in the right group. The difference was statistically significant（χ2=4.69，P0.05）. Conclusion The improved method was more convenient in operation and less tumor cells spreading in establishment of bone tumor model compared with the traditional method. 　　Key words：VX2 tumor；Bone tumor；Model