松针油对人胃癌SGC―7901细胞增殖影响.docVIP

松针油对人胃癌SGC―7901细胞增殖影响 　　[摘要]目的 研究松?油对人胃癌SGC-7901细胞增殖的影响，为揭示松针油的抗胃癌作用机制提供理论和实验基础。方法 采用水蒸气蒸馏法提取松针油。向对数生长期的人胃癌SGC-7901细胞中加入等体积不同浓度的松针油，培养24 h后进行细胞形态学观察；采用MTT法检测松针油对胃癌SGC-7901细胞增殖的影响，将对数期细胞分为阴性对照组、不同浓度给药组，加入不同浓度的松针油，培养24 h后每孔加MTT 20 μL，37℃孵育4 h，每孔加入0.04 mol/L DMSO 200 μL，用酶联免疫检测仪在490 nm波长测光密度值（OD值）。 结果 松针油给药浓度越高，存活细胞越少，细胞发生体积变小、变圆、核固缩等形态学变化；MTT实验结果表明：与对照组的OD值相比较，1∶200、1∶300和1∶400给药组的OD值明显下降（P0.001），1∶500给药组的OD值也有下降，差异有统计学意义（P0.01）；与对照组的细胞存活率相比较，1∶200、1∶300、1∶400和1∶500给药组细胞存活率明显下降，差异有统计学意义（P0.001），半数抑制浓度（IC50）为1.9 mg/mL。 结论 松针油对胃癌SGC-7901细胞增殖有抑制作用，且随松针油浓度升高抑制作用明显增强。 　　[关键词] 松针油；胃癌；细胞增殖；MTT法 　　[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-9701（2018）03-0001-04 　　Influence of essential oil extracted from pine needles on cell proliferation of human gastric cancer cell line SGC-7901 　　WU Dan MENG Nana HAN Yang LI Lingyu TANG Chunyin 　　Mudanjiang Medical University， Mudanjiang 157011， China 　　[Abstract] Objective To investigate the influence of essential oil extracted from pine needles on cell proliferation of human gastric cancer cell line SGC-7901， and to provide a theoretical and experimental basis for revealing the mechanism of anticancer effects of essential oil extracted from pine needles against gastric cancer. Methods Essential oil from pine needles was collected by steam distillation. Equal volume of oil with different concentrations was added into human gastric cancer cell line SGC-7901 during its logarithmic phase， cell morphology was observed after 24 h of cell culture； cell proliferation was detected by MTT assay， cells in logarithmic phase were divided into negative control group and different concentrations groups， oil with different concentrations was added， after 24 hours of cell culture， 20 μL MTT was added into each hole， then after 4 hours of incubation in 37℃， 200 μL of 0.04 mol/L DMSO was added into each hole， optical density（OD） at 490 nm was measured by enzyme-linked immunosorbent assay. Results The higher the concentration of essential oil extracted fro