氨茶碱对重症胎粪吸入新生兔TNFα和IL8水平影响.docVIP

氨茶碱对重症胎粪吸入新生兔TNFα和IL8水平影响 　　[摘要] 目的：观察氨茶碱对重症胎粪吸入新生兔的肿瘤坏死因子-α（TNF-α）和白细胞介素-8（IL-8）的影响。方法：将24只新生兔随机分成灌入胎粪后实施同步间歇指令通气（SIMV）应用氨茶碱组（氨茶碱组，n=6）、灌入胎粪后实施同步间歇指令通气组（氨茶碱对照组，n=6）、胎粪灌入后不予通气组（通气对照组，n=6）以及灌生理盐水不予通气组（空白组，n=6）。氨茶碱对照组和氨茶碱组给予机械通气6 h。氨茶碱组在胎粪灌注后0.5 h和2.5 h后静脉给予氨茶碱（2.0 mg/kg），并用酶联免疫吸附试验（ELISA法）测定肺组织匀浆液及支气管肺泡灌洗液（BALF）中TNF-α和IL-8含量。结果：氨茶碱组和氨茶碱对照组比较肺组织匀浆和BALF中TNF-α和IL-8含量降低，差异均有统计学意义（均P＜0.05）。与通气对照组、空白组比较，氨茶碱对照组、氨茶碱组的肺组织匀浆和BALF中TNF-α和IL-8含量明显升高，差异均有统计学意义（均P＜0.05）。结论：氨茶碱可以降低新生兔重症胎粪吸入肺组织匀浆和BALF中TNF-α和IL-8的水平。 　　[关键词] 胎粪吸入；肿瘤坏死因子-α；白细胞介素-8；氨茶碱；新生兔 　　[中图分类号] R722.12；R965 [文献标识码]A [文章编号]1673-7210（2011）03（c）-020-03 　　 　　Effects of Aminophylline on TNF-α and IL-8 levels of newborn rabbits with severe meconium aspiration 　　ZHAN Feng1, WU Rong1, JIN Guihong1, ZHOU Haiyan1, LI Na1, HU Bo2 　　1.Department of Pediatrics, Chuzhou Clinical College, Anhui Medical University, Chuzhou 239000, China; 2.Department of Pediatrics, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China 　　[Abstract] Objective: To observe effect Aminophylline on the levels of the tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) of newborn rabbits with severe meconium aspiration. Methods: 24 newborn rabbits were randomly divided into four groups: implement synchronized intermittent mandatory ventilation (SIMV) after meconium instillation treated with Aminophylline (Aminophylline group, n=6), implement synchronized intermittent mandatory ventilation after meconium instillation (Aminophylline control group, n=6), no ventilation after meconium instillation (ventilation control group, n=6) and the saline irrigation with no ventilation group (control group, n=6). Aminophylline control group and Aminophylline group received 6 hours of mechanical ventilation. Rabbits intravenously received Aminophylline (2.0 mg/kg) at two doses 0.5 h and 2.5 h after meconium instillation in Aminophylline group. Enzyme-linked immunosorbent assay (ELISA) was used to measure TNF-α and IL-8 in lung homog