淀粉芽孢杆菌HAB―7对18株植物病原真菌抑制作用.docVIP

淀粉芽孢杆菌HAB―7对18株植物病原真菌抑制作用 　　摘 要 HAB-7是一株分?x自豇豆根际土壤、具有较强抑菌作用的解淀粉芽孢杆菌（Bacillus amyloliquefaciens）。研究HAB-7对18株植物病原真菌及其发酵液粗提蛋白对植物病原的抑制作用，并测定其对番茄种子的促生作用。采用平板对峙法测定其对18株植物病原真菌的抑制活性，以橡胶树炭疽菌为指示菌，对其发酵上清液经60%、80%硫酸铵盐析获得的抗菌粗蛋白进行测试；并将HAB-7菌液稀释成6个浓度梯度，分别用来对番茄种子进行促生测试。结果显示：HAB-7对18株植物病原真菌的平均抑菌率为61.03%；其发酵上清液对橡胶树炭疽病菌的抑制率为56.80%，80%硫酸铵粗提蛋白对橡胶树炭疽病菌的抑制率为38.88%；10-4 CFU/mL的HAB-7菌液浓度促进了番茄种子胚根的伸长，长度比CK提高67.76%。HAB-7菌株对植物病原菌有较广谱的抑菌活性，其分泌的活性物质除了活性蛋白外，可能还有其他的活性物质，低浓度的HAB-7菌液能促进植物根系生长。本研究结果为后续研究其致病机理起到重要的作用。 　　关键词 解淀粉芽孢杆菌 ；抑菌活性 ；抑真菌蛋白 ；促生作用 　　中图分类号 S432 文献标识码 A Doi：10.12008/j.issn.1009-2196.2017.02.013 　　Analysis of Antifungal Activity of Bacillus amyloliquefaciens 　　HAB-7 Against 18 Phytopathogens 　　LIU Wenbo1） QIN Chunxiu1） XIONG Yanhong1） 　　SUN Liang2） LIN Chunhua1） MIU Weiguo1） ZHENG Fucong1） 　　（1 Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/ 　　College of Environment and Plant Protection，Hainan University， Haikou， Hainan 570228， China； 　　2 Rubber Research Institute， CATAS， Danzhou， Hainan 571737，China） 　　Abstract HAB-7 is a strain of Bacillus amyloliquefaciens isolated from the rhizosphere of Vigna unguiculata and has a strong inhibitory effect on phytopathogen. The strain HAB-7 was tested against 18 phytopathogenic fungi， and the crude protein extracted from the fermented HAB-7 was also tested against the phytopathogens to observe their inhibitory activities. The tomato seeds were treated with the strain HAB-7 to observe their germination and growth. Dural culture assay was used to test the antibacterial activity of HAB-7 against 18 plant pathogens. The antifungal crude protein extracted from the supernatant of fermented HAB-7 via precipitation of 60% or 80% ammonium sulfate was tested against the fungi of rubber anthracnose caused by Colletotrichum gloeosporioides Penz. CGR-1 strain which was used as an indicator pathogen. The HAB-7 was diluted into 6 different concentrations， with which tomato seeds were treated to observe their germination and