消痰散结方对MKN―45人胃癌裸鼠原位移植瘤细胞超微结构影响.docVIP

消痰散结方对MKN―45人胃癌裸鼠原位移植瘤细胞超微结构影响 　　摘要：目的 观察消痰散结方对MKN-45人胃癌裸鼠原位移植瘤细胞超微结构的影响，探讨其治疗胃癌的作用机制。方法 采用MKN-45人胃癌细胞株建立裸鼠皮下瘤模型皮下传3代作为实验模型的瘤源，采用0B胶黏合法建立人胃癌裸鼠原位移植瘤模型。40只模型裸鼠分为空白对照组、模型组、消痰散结方组、替加氟组，每组10只。消痰散结方组给予消痰散结方灌胃，替加氟组给予替加氟灌胃，模型组给予生理盐水灌胃，空白对照组正常饮食喂养。干预6周后，测瘤质量，计算抑瘤率。透射电镜观察药物作用后移植瘤细胞超微结构。结果 消痰散结方组瘤质量明显低于空白对照组及模型组（P0.05，P0.01），其抑瘤率为46.2%；电镜观察可见胃癌细胞出现典型细胞凋亡的形态学改变。结论 消痰散结方具有诱导MKN-45人胃癌裸鼠原位移植瘤细胞凋亡的作用，提示诱导细胞凋亡是消痰散结方治疗胃癌的可能作用机制之一。 　　关键词：消痰散结方；胃癌；细胞超微结构；小鼠 　　DOI：10.3969/j.issn.1005-5304.2015.09.017 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）09-0060-03 　　Effects of Xiaotan Sanjie Decoction on Ultramicrostructure in MKN-45 Human Gastric Cancer Orthotopic Xenograft YE Min， SUN Da-zhi， XIU Li-juan， LU Ye， Instructor：WEI Pin-kang （Department of Traditional Chinese Medicine， Shanghai Changzheng Hospital， Second Military Medical University of PLA， Shanghai 200003， China） 　　Abstract：Objective To observe the effects of Xiaotan Sanjie Decoction on ultramicrostructure in MKN-45 human gastric cancer orthotopic xenograft；To discuss its mechanism for gastric cancer. Methods MKN-45 human gastric cancer cell line was used to establish subcutaneous tumor model in nude mice as an experimental model which passed three generations of tumor source. Then OB glue was used to establish human gastric cancer orthotopic xenograft model. 40 model nude mice were divided into control group， model group （received gavage with saline）， Xiaotan Sanjie Decoction group （received garage with Xiaotan Sanjie Decoction） and Tegafur group （received gavage with Tegafur）， 10 mice in each group. Mice in the control group were under normal diet， without medicine intervention. After 6 weeks of treatment intervention， tumor weight was detected， inhibitory rate was calculated， the ultrastructural changes of gastric cancer cells were detected through transmission electron microscopy. Results The tumor weight in Xiaotan Sanjie Decoction group was significantly lower than that in control group and model group （P0.05，P0.01）. The