白H合剂对体外培养人永生化角质形成细胞分泌血管内皮生长因子及其受体2表达影响.docVIP

白H合剂对体外培养人永生化角质形成细胞分泌血管内皮生长因子及其受体2表达影响 　　摘要：目的 观察中药白?H合剂干预人永生化角质形成细胞（HaCaT细胞）后，对其血管内皮生长因子（VEGF）的分泌量及其受体2（VEGFR-2）基因和蛋白表达的影响，并探讨其作用机制。方法 体外培养HaCaT细胞建立银屑病实验模型。SD大鼠随机分为空白血清组、阿维A组和白?H合剂低、中、高剂量组，并单设空白对照组。给药后制备血清，干预HaCaT细胞模型。ELISA检测VEGF分泌量，RT-PCR检测VEGFR-2基因表达，Western blot检测VEGFR-2蛋白表达。结果 与空白对照组比较，白?H合剂低、中、高剂量组干预HaCaT细胞后，VEGF分泌量及VEGFR-2基因和蛋白表达均明显抑制，且呈浓度依赖性。结论 白?H合剂可能通过抑制VEGF分泌量、降低VEGFR-2基因和蛋白表达以达到治疗银屑病的作用。 　　关键词：白?H合剂；人永生化角质形成细胞；血管内皮生长因子；血管内皮生长因子受体-2；大鼠 　　DOI：10.3969/j.issn.1005-5304.2015.11.020 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）11-0073-03 　　Effects of White Mange Mixture on VEGF Content and Genetic and Protein Expressions of VEGFR-2 in Cultured HaCaT Cells CHEN Xing， LI Xin-hong， LU Yi-ping （Affiliated Hospital of Liaoning University of Traditional Chinese Medicine， Shenyang 110032， China） 　　Abstract：Objective To observe the effects of White Mange Mixture on the content of VEGF and the genetic and protein expressions of VEGFR-2 of HaCaT cells；To explore its mechanism. Methods HaCaT cells were cultured in vitro to establish psoriasis experimental model. SD rats were randomly divided into the blank serum group， white mange mixture of low-， medium- and high- dose groups and acitretin group， meanwhile to set up blank control group. Cultured HaCaT cells model were intervened with different medicine serum. ELISA was applied to detect the content of VEGF. RT-PCR and Western blot were used to detect the genetic and protein expressions of VEGFR-2. Results Compared with the blank control group， White Mange Mixture of low-， medium- and high-dose groups interventing HaCaT cells could obviously inhibit the secretion of VEGF and decrease the genetic and protein expression of VEGFR-2 in a dose-dependent manner. Conclusion White Mange Mixture plays a role in the treatment of psoriasis possibly through inhibiting the content of VEGF and decreasing the genetic and protein expressions of VEGFR-2. 　　Key words：White Mange Mixture；HaCaT cells；VEGF；VEGFR-2；rats 　　血管生成是银屑病发生发展的