疏肝健脾活血方对肝郁脾虚证FD大鼠血浆NTCRHSS影响.docVIP

疏肝健脾活血方对肝郁脾虚证FD大鼠血浆NTCRHSS影响 　　【摘要】 目的：研究疏肝健脾活血方对肝郁脾虚证功能性消化不良（functional dyspepsia，FD）大鼠血浆神经降压素（neurotensin，NT）、促肾上腺皮质激素释放激素（corticotropin releasinghormone，CRH）、生长抑素（Somatostatin，SS）的影响，探讨其作用机制。方法：70只SPF级雌性SD大鼠按体质量随机抽取10只作为正常组，其余60只采用“慢性束缚+过度疲劳+饮食失节”复合因素法造模21 d，造模成功后，按体质量随机分为模型组、疏肝健脾活血方高剂量组、中剂量组、低剂量组、柴芍六君子汤组、多潘立酮组。分组后，正常组和模型组大鼠灌胃给予相应体积纯净水，其他大鼠灌胃给予相应药物干预14 d。实验结束后，ELISA法检测血浆NT、CRH、SS含量。结果：与正常组比较，模型组大鼠血浆NT含量显著下降（P0.05）；多潘立酮组、疏肝健脾活血方高剂量组、疏肝健脾活血方中剂量组、疏肝健脾活血方低剂量组大鼠血浆CRH含量显著下降（P0.05）；多潘立酮组、柴芍六君子组、疏肝健脾活血方各剂量组大鼠血浆SS含量显著增加（P0.05或P0.01）。结论：疏肝健脾活血方能够调节肝郁脾虚型FD大鼠血浆NT、SS、CRH表达，这可能是其作用机制之一。 　　【关键词】 中医中药； 功能性消化不良； 疏肝健脾活血方； 神?降压素； 促肾上腺皮质激素释放激素； 生长抑素 　　【Abstract】 Objective：To observe the effect of Shu Gan Jian Pi Huo Xue（SGJPHX） decoction on NT，CRH，SS in plasma of FD rats with liver depression and spleen deficiency.Method：70 SPF female rats were randomly divided into normal group（10 rats） and modeling group（60 rats） according to the body weight of rats.The modeling group rats were adopted by “chronic restraint stress + excess fatigue + improper” diet for 21 d to form the model.After modeling，the modeling group rats were randomly divided into model group，SGJPHX high dose group， medium dose group，low dose group，Chai Shao Liu Jun Zi （CSLJZ） decoction group and domperidone group.After group division，the SGJPHX high dose group，medium dose group and low dose group rats were given SGJPHX decoction，the CSLJZ group rats were given CSLJZ decoction，the domperidone group rats were given domperidone，and the model group and normal group were given pure water for 14 d.The NT，CRH，SS in plasma of FD Rats were detected by ELISA.Result：Compared with the normal group，the content of NT in model group was decreased and CRH was increased （P0.05）.The content of CRH in SGJPHX high dose group，medium dose group，low dose group and domperidone group were significantly reduced（P 　　【Key words】 Traditional Chinese medicine； Functional dyspepsia； SGJPHX decoction； Neurotensin； Corticotropin releasinghormone； So