竹节参总提物对D半乳糖致衰老小鼠影响.docVIP

竹节参总提物对D半乳糖致衰老小鼠影响 　　摘要：目的观察竹节参总提物对衰老小鼠的学习记忆、抗氧化及抗凋亡的影响,并探讨其作用机制。方法雄性昆明种小鼠随机分为正常组、模型组、维生素E组和竹节参低、高剂量组。除正常组外,其余各组颈背部皮下注射D-半乳糖建立衰老模型。各给药组分别给予竹节参总提物和维生素E灌胃,正常组和模型组给予等量生理盐水灌胃,1次/d,连续7周。第8周进行小鼠学习和记忆能力测试。测试结束后,苏木素-伊红染色观察海马CA1区神经元的形态变化,生化法检测脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)水平,RT-PCR检测脑组织中Bcl-2、Bax mRNA表达。结果竹节参低、高剂量组均能缩短小鼠寻找平台时间,改善小鼠学习记忆能力；显著增强SOD、GSH-Px活性,降低MDA含量；升高Bcl-2 mRNA、降低Bax mRNA表达水平。结论竹节参总提物具有抗衰老的作用。 　　关键词：竹节参总提物；D-半乳糖；抗衰老；抗氧化；小鼠 　　DOI：10.3969/j.issn.1005-5304.2014.07.010 　　中图分类号：R285.5文献标识码：A文章编号：1005-5304(2014)07-0032-04 　　Effects of Total Panax Japonicus Extract on D-galactose Induced Aging MiceWAN Jing-zhi, YUAN Ding, DI Guo-jie, ZHANG Chang-cheng, LIU Chao-qi, ZHOU Zhi-yong, WANG Ting (Medical College of China Three Gorges University, Yichang 443002, China) 　　Abstract：Objective To research the protective effects of total Panax Japonicus extract on learning memory, antioxidation, and anti-apoptosis of aging mice, and explore the mechanism. Methods Male mice were randomly divided into normal group, model group, Vitamin E (VE) group, Panax Japonicus extract low and high dose group. Except for the normal group, the other groups were injected with D-gal on the back of the neck subcutaneously to establish aging model. Normal group and model group were given a gavage with saline and each treatment group was given a gavage with total Panax Japonicus extract and VE once a day for 7 weeks after the aging model established. All mice were be measured their learning and memory ability in the eighth week. After the test, the morphological changes of CA1 neurons were observed by HE stain. SOD, GSH-Px, MDA levels in brain tissue were measured by biochemical method, the expressions of Bcl-2 and Bax mRNA were evaluated by RT-PCR. Results Mice in Panax Japonicus extract low and high dose group could spend less time in searching for the platform, improve the learning and memory ability. Total Panax Japonicus extract increased the activity of SOD and GSH-Px, while