竹荪水提物对路易斯肺癌细胞抑制效应研究.docVIP

竹荪水提物对路易斯肺癌细胞抑制效应研究 　　[摘要] 目的 探讨竹荪水提物对肺癌细胞的抑制效应。 方法 选取路易斯肺癌细胞（LLC）为靶细胞，接种于48孔或96孔板，接种密度分别为2×104/孔和1×104/孔，加入不同浓度的竹荪水提物：0、1、2 mg/mL，培养1～3 d，分别采用镜下观察、细胞计数试剂盒（CCK-8）、流式细胞学和细胞实时分析系统（RTCA）检测细胞生长状态。 结果 镜下直接观察，与对照组比较，竹荪水提物处理组LLC细胞数量明显较少，高浓度组细胞生长状态不佳；CCK-8检测结果显示，高浓度组OD450为0.24，仅为对照组的一半（0.59），细胞增殖受到明显抑制，抑制率为59%（P 0.05）；流式细胞学检测显示，经竹荪水提物处理后，LLC早期凋亡比例由6%上升到48%，凋亡及死亡细胞比例由41%上升到68%；RTCA实时监测数据显示，竹荪水提物高浓度处理组LLC生长的电阻值为0，低浓度组为0.4，对照组为3.7，细胞贴壁生长状态呈剂量-效应关系。 结论 竹荪水提物可以抑制LLC生长。 　　[关键词] 竹荪；水提物；LLC；增殖；抑制 　　[中图分类号] R734.2[文献标识码] A[文章编号] 1673-7210（2014）06（a）-0009-04 　　 　　Inhibition effect study of aqueous extract from dictyophora indusiata on lewis lung cancer cell 　　WANG Xiaohong1 JIANG Hong2 YU Xinmin1 　　1.Department of Chemotherapy, Zhejiang Provincial Cancer Hospital, Zhejiang Province, Hangzhou 310022, China; 2.Department of Thoracic Surgery, the First Peoples Hospital of Hangzhou City, Zhejiang Province, Hangzhou 310006, China 　　[Abstract] Objective To study the inhibition effect of aqueous extract from Dictyophora indusiata on Lewis lung cancer cell. Methods LLCs were seeded in 48-well or 96-well plate, with each well containing 2×104/well and 1×104/well, respectively. Different concentration aqueous extract from Dictyophora indusiata (DI) (0, 1, 2 mg/mL) were added into culture system, after 1-3 days, the cell status, proliferation and apoptosis were detected by microscopy, CCK-8 kit, flow cytometry and real time cell analysis system. Results Comparison to control group, the cell number of DI treated group obviously decreased. Furthermore, the cell status was weak in high dose group. The OD450 value of high dose group (0.24) was only half of the control group (0.59) in the CCK-8 experiment, and it indicated that the cell proliferation was inhibited with the inhibited rate of 59% (P 0.05). In flow cytometry analysis, the apoptosis in early stage of control group was 6%, while it increased to 48% in DI treated group. The total death cell from 41% increased to 68% after DI tr