纤维素分解菌对堆肥有机质全碳及纤维素降解率影响.docVIP

纤维素分解菌对堆肥有机质全碳及纤维素降解率影响 　　摘要从堆肥、牛粪、秸秆堆腐物中分离4株纤维素分解菌。在纤维素粉及刚果红培养基中筛选出3株生长速度快、透明圈出现早且透明圈直径与菌落直径比值大的菌株,分别为FDQ1、FDQ2、FDQ3。不同菌株混合培养较单独培养酶活力提高,表明菌株间有协同作用。FDQ1+FDQ2和FDQ1+FDQ3菌株等比例混合进行牛粪与鸡粪混合堆肥,结束时有机质分别下降19.57%和18.40%,对照下降6.58%;全碳量分别为31.33%、32.42%,对照为41.20%,较发酵初期降低13.80%、12.71%和3.93%;纤维素分解率较对照提高95.12%和78.61%,表明纤维素分解菌在加快纤维素降解的同时还促进了碳素矿化。 　　关键词纤维素分解菌;酶活;有机质;全碳;纤维素降解率 　　中图分类号S182文献标识码A文章编号 1007-5739(2009)22-0260-02 　　 　　EffectofCellulose-DecomposingMicroorganismsonOrganicMatter,Total CarbonandDegradationRateofCellulose 　　YU Ting-qiaoXU Feng-hua *SHI Shan-shanGUO Mei-xian 　　(School ofResources Environment,Northeast Agriculture University,Harbin Heilongjiang 150030) 　　Abstract4 strains of cellulose-decomposing microorganisms were isolated from composting,cattle manure and straw filth. FDQ1,FDQ2 and FDQ3,which had fast growth rate,earlier transparent circle,bigger ratio oftransparent circle diameter and colony diameter,were screened from cellulose and congo red medium. Enzyme activity of mixed culture was increased compared with single culture,which showed that strains had synergistic effect. Mixing FDQ1+FDQ2,FDQ1+FDQ3 with chicken and cattle manure in equal ratio,it showed that organic matter decreased by 19.57% and 18.40% after compost,the control decreased by 6.58%;total carbon contents were 31.33% and 32.42%,respectively,the control was 41.20%,which decreased by 13.80%,12.71% and 3.93% compared with the early stage of the fermentation;degradation rate of cellulose increased by 95.12% and 78.61% compared with the control. It was concluded that cellulose-decomposing microorganisms could acclerate cellulose degradation and promote carbon mineralization. 　　Key wordscellulose-decomposing microorganism;enzyme activity;organic matter;total carbon;degradation rate ofcellulose 　　 　　纤维素是可无限再生的天然资源,我国每年约有6亿t作物秸秆,利用纤维素分解菌降解纤维素实现秸秆资源化是一个备受关注的问题。现研究牛粪与鸡粪混合堆肥时添加纤维素分解菌对有机质、全碳及纤维素的影响,以期为纤维素分解菌的应用提供参考。 　　1材料与方法 　　1.1试验材料 　　原料:牛粪、秸秆堆腐物、稻壳粉;培养基:纤维素粉、刚果红;菌株来源:堆肥、牛粪、秸秆堆腐物分离的纤维素分解菌。