紫外分光光度法与高效液相色谱法测定板栗花水提液中总黄酮含量比较研究.docVIP

紫外分光光度法与高效液相色谱法测定板栗花水提液中总黄酮含量比较研究 　　摘要 [目的]建立板栗花水提液中总黄酮含量的快速、准确测定方法，为产品开发提供技术支持。[方法]以槲皮苷为对照品，分别采用紫外分光光度法与高效液相色谱法测定板栗花水提液中总黄酮的含量。[结果]紫外分光光度法测得总黄酮的含量为4.62 mg/mL，高效液相色谱法测得总黄酮的含量为4.64 mg/mL，2种方法所得结果基本一致。[结论]紫外分光光度法与高效液相色谱法均可用于板栗花水提液中总黄酮的准确定量，但在实际生产应用中完全可用紫外分光光度法替代高效液相色谱法对总黄酮的含量进行快速测定。 　　关键词 板栗花水提液；总黄酮；紫外分光光度法；高效液相色谱法 　　中图分类号 TS201 文献标识码 A 文章编号 1007-5739（2018）02-0255-03 　　Comparison of Quantitative Determination of Total Flavonoids in Water Extracts of Chestnut Flower by UV Spectrophotometry and HPLC 　　WANG Feng-chun 1 ZENG Yan-ling 2 　　（1 Research and Development Center of Chestnut Industry of Qianxi County，Qianxi Hebei 064300； 2 School of Chinese Materia Medica， 　　Beijing University of Chinese Medicine） 　　Abstract [Objective]To establish rapid and accurate quantitative determination method of total flavonoids in water extracts of chestnut flower and provide technical support for product development.[Methods]Taking quercitrin as the contool，UV spectrophotometry and HPLC were used to determine the content of total flavonods in water extracts of chestnut flower，respectively.[Results]The content of total flavonoids determined by UV spectrophotometry was 4.62 mg/mL，and by HPLC was 4.64 mg/mL.The content of total flavonoids obtained by the two methods were basically consistent.[Conclusion] Both UV spectrophotometry and HPLC could be used for accurate quantitative determination of total flavonoids in water extracts of chestnut flower，but in actual production，UV spectrophotometry could completely replace HPLC to determine the total flavonoids quickly. 　　Key words water extracts of chestnut flower；total flavonoids；UV spectrophotometry；HPLC 　　板栗花?榭嵌房疲?Fagaceae）栗属植物板栗（Castanea m-ollissima Bl.）的雄性花序，具有涩肠止泻的功效[1]，民间用其水煎剂治久治不愈的细菌性痢疾，疗效较好。已有研究表明，板栗花主要含黄酮[2]、多酚[3]、挥发油[4]、木脂素[5]及甾体[6]等多类化学成分，其中黄酮（苷）类成分的含量最高。已报道的黄酮类化合物包括栗黄酮苷A、栗黄酮苷B、槲皮素（querc-etin）、槲皮素-3-O-β-D-半乳糖苷（quercetin-3-O-β-D-ga-lactopyranoside）、槲皮素-3-O-（6-O-没食子酰基）-β-D-半乳糖苷[quercetin-3-O-（6-O-galloyl）-β-D-galactoside]、槲