ω 3不饱和脂肪酸对视网膜新生血管作用的实验研究-眼科学专业论文.docxVIP

II II Abstract Object: T0 explore the effect of ω-3 PUFAs on the retinal neovascularrization． Methods: Thirty two 7 days old clearing Wistar pups with 4 mother mice were randomly divided into four groups to make Retinopathy of Premature (ROP) model:control group(A),low dose experimental group(B)、middle dose experimental group(C) and high dose experimental group(D), each group was with a mother mice , and experimental group was feded by different dose of ω-3PUFAs as 2.5mg/kg/d、 7.5mg/kg/d and 15mg/kg/d. The numbers of endotheliocyte nuclei of neovascularization which broke through the retinal inner limiting memberane was detcted by immunohistochemistry,the expression of Regulators of G—protein Signaling（RGS5）in retinal was detected by Western blot. Results: The numbers of endotheliocyte nuclei of neovascularization which broke through the retinal inner limiting memberane were( 17.00±3.42）、 （15.63±2.67）、 （9.13±1.96）、（8.38±2.19）in four groups respecitively. The ration between RGS5 and β-Actin were( 0.24±0.96）、 （0.36±0.08）、（0.51±0.23）、（0.66±0.19）in four groups respecitively.There was significan difference found between control group and middle dose experimental group(P0．001),also there were difference for low dose experimental group and high dose experimental group compared with the control group(P0．05).but there was no statistical differencewan found between middle dose experimental group and high dose experimental group(P0．05). Conclusion: RGS5 take a part in retinal neovascularrization , The dose of ω-3 PUFAs take effect on RGS5, and had a protective effect against pathological angiogenesis in retinal. Key words: ω-3 PUFAs；Retinal；Neovascularrization III III 主要符号表 英文缩写 英文全称 中文译文 ROP Retinopathy of prematurity 早产儿视网膜病变 PUFAs DHA Polyun s a tur a te d f a tty Docosahe xaenoicacid a c ids 多不饱和脂肪酸 二十二碳六烯酸 EPA Eicosapentaenoic acid 二十碳五烯酸 RGS regulators of G—protein signaling G 蛋白信号调节子 GPCR G—protein—coupled receptors G 蛋白偶联受体 VEGF Vascular endothelial growth