脑源性神经营养因子Val66Met多态性与脑出血关联分析.docVIP

脑源性神经营养因子Val66Met多态性与脑出血关联分析 　　[摘要] 目的 探讨脑源性神经营养因子Val66Met多态性与脑出血的关系。 方法 采用限制性内切酶片段长度多态性技术检测脑源性神经营养因子Val66Met多态性，在110例脑出血患者和101名健康对照组中的分布；比较病例组、对照组基因型、等位基因分布差异。 结果 （1）脑出血组和对照组A基因型分布频率分别为0.40和0.20，显著高于对照组，差异具有统计学意义（x2=13.81，P=0.001）； （2）两组等位基因分布频率差异也具有统计学意义，差异具有统计学意义（x2 =13.12，P=0.000）。 结论 脑源性神经营养因子Val66Met多态性的A等位基因同脑出血相关，可能该病的易感基因。 　　[关键词] 脑源性神经营养因子；Val66Met；多态性；脑出血 　　[中图分类号] R741 [文献标识码] A [文章编号] 2095-0616（2014）04-62-03 　　Positive association of brain-derived neurotrophic factor（BDNF） gene Val66Met polymorphism with cerebral hemorrhage 　　LI Jifeng1 WANG Na2 　　1.Department of Neurology，Dengfeng Peoples Hospital， Dengfeng 452470，China；2.Department of Psychiatry，Zhengzhou Ninth Peoples Hospital，Zhengzhou 450053，China 　　[Abstract] Objective To explore the possible association between Val66Met polymorphism of BDNF and cerebral hemorrhage in Chinese Han population. Methods We analyzed polymorphisms of the BDNF Val66Met polymorphism with a lfragmentation length polymorphism（RFLP）-based method in 110 patients with cerebral hemorrhage and 101 unrelated healthy control subjects. Results （1）The AA genotypes frequencies of Val66Met polymorphism in cerebral hemorrhage patients and control subjects were 0.40 and 0.20，respectively，the difference was statistic（x2=13.81，P=0.001） （2）The allele frequencies of Val66Met polymorphism in cerebral hemorrhage and control group were significant（x2=13.12，P=0.000）.And the allele A frequency of Val66Met in cerebral hemorrhage patients was significantly higher than that of control subjects （P0.01）. Conclusion This study suggests that the BDNF Val66Met may be the major risk factors in increasing susceptibility to cerebral hemorrhage in Chinese Han population. 　　[Key words] Brain-derived neurotrophic factor（BDNF） gene；Val66Met；Polymorphism；Cerebral hemorrhage 　　既往的研究表明，脑出血（intracerebral hemorrhage，ICH）后，脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）的表达显著增高，但是其机制尚不完全明了[1-2]。在BDNF基因众多的单核苷酸多态性位点中，位于第五外显子区域的Val66Met多态性同调节BDNF的表达密切相关，但该基因