电针调整慢性疲劳大鼠自发活动的中枢机制研究-针灸推拿学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 10 ABSTRACT Objective: Observe electro-acupuncture’s effect on chronic fatigue rat’s exhaustive swimming time、finding the platform time, spontaneous activity of 24 hours, supra chiasmatic neclue’s γ － aminobutyric acid’s expression and pineal gland’s melatonion positive cell expression’s circadian rhythms, to reveal the mechanism of that how electro-acupuncture adjusts chronic fatigue rat’s spontaneous activity ‘s circadian rhythms. Methods: Divide SD rats of clean grade to normal control group、model group、model electro-acupuncture group、model Sham injection group、model CRF injection group、 model CRF injection and electro-acupuncture group、model Antagonist-CRH injection group and model Antagonist-CRF injection and electro-acupuncture group randomly, make chronic fatigue model by chronic restraint and cold water swimming.Treat model Sham injection group、model CRF injection group、model CRF injection and electro-acupuncture group 、 model Antagonist-CRH injection group 、 model Antagonist-CRF injection and electro-acupuncture group with intracerebroventricular administration of surgery separately，while treat model electro-acupuncture group、 model CRF injection and electro-acupuncture group、model Antagonist-CRF injection and electro-acupuncture group with electro-acupuncture on BL23、ST36 . Observe and record the exhaustive swimming time、finding the platform time and spontaneous activity of 24 hours of all groups. Observe and record supra chiasmatic neclue’s γ－aminobutyric acid’s and melatonion receotor positive cell’s expression at 0:00、4:00、8:00、12:00、16:00、20:00 by immunohistochemistry. Results: 1.Exhaustive swimming time and finding the platform time after treatment: exhaustive swimming time after treatment,model group compared with normal control group time to be shortened，finding the platform time conversely.Exhaustive swimming time of model electro-acupuncture group extended compared with model group,finding