重组人甲状旁腺素134对去卵巢大鼠骨质疏松作用研究.docVIP

重组人甲状旁腺素134对去卵巢大鼠骨质疏松作用研究 　　[摘要] 目的 探讨重组人甲状旁腺素1-34（hPTH1-34）对去卵巢大鼠骨质疏松的治疗作用。 方法50只7个月龄SD健康雌性大鼠，30只行双侧卵巢摘除术，20只做假手术（SHAM），12周后各处死10只证实骨质疏松造模成功。余20只双侧卵巢摘除大鼠随机分为去卵巢（OVX）安慰剂组10只和hPTH1-34治疗组10只，10只SHAM组作对照。hPTH1-34治疗组剂量为20 μg/kg，皮下注射，每周注射3次。OVX安慰剂和SHAM组皮下注射0.5 mL生理盐水，每周注射3次。治疗3个月后，测定并比较三组股骨、腰椎骨密度（BMD）、血清钙、磷、骨源性碱性磷酸酶（BALP）、抗酒石酸酸性磷酸酶-5b（Trap-5b）水平。 结果 hPTH1-34治疗组股骨和腰椎BMD较OVX安慰剂组明显升高[（0.151±0.008）g/cm2比（0.135±0.013）g/cm2，（0.181±0.010）g/cm2比（0.159±0.008）g/cm2，P 0.05）。 结论 hPTH1-34通过刺激成骨细胞的活性，抑制破骨细胞，可显著增加BMD，对去卵巢大鼠骨质疏松有效。 　　[关键词] 重组人甲状旁腺素1-34；骨质疏松症；骨密度；骨转换指标 　　[中图分类号] R580[文献标识码] A[文章编号] 1673-7210（2014）06（b）-0017-04 　　 　　Effect of human parathyroid hormone (hPTHl-34) on osteoporosis of ovariectomized rats 　　CHEN Bo1 LI Cuiqiong1 ZHU Changqing1 LI Wanmei1 CAO Xiaozhen2 　　1.Department of Endocrinology, the 12th People′s Hospital of Guangzhou City, Guangdong Province, Guangzhou 510620, China; 2.Department of Ultrasonography, the 12th People′s Hospital of Guangzhou City, Guangdong Province, Guangzhou 510620, China 　　[Abstract] Objective To investigate the effects of human parathyroid hormone (hPTHl-34) on the osteoporosis of ovariectomized rats. Methods 50 female 7-month-old SD rats were used for this experiment and 30 of them were ovariectomized and another 20 received sham operation. After 12 weeks of ovariectomy the osteoporosis model was confirmed by use of 10 ovariectomized and sham-operated rats. The osteoporotic rats of 20 were randomly divided into 2 treatment groups: hPTHl-34 and OVX placebo groups with 10 rats in each group, the other 10 rats were sham-operated as the control for SHAM group. Totally hPTHl-34 (20 μg/kg) were subcutaneous injected into the rats of hPTHl-34 group once every other day. The rats of the OVX placebo group and SHAM group were injected with 0.5 mL saline once every other day. The bone mineral density (BMD), serurn Ca, P, bone alkaline phosphatase (BALP), serum tartrate-resistant acid phosphatase 5b (Trap-5b) were measured after 3 months