阿尔茨海默病患者血清氧化应激相关指标及Aβ水平测定临床意义.docVIP

阿尔茨海默病患者血清氧化应激相关指标及Aβ水平测定临床意义 　　[摘要] 目的 探?阿尔茨海默病（AD）患者血清中氧化应激相关指标及Aβ沉积相关指标在诊疗过程中的临床意义。方法 选取抚顺矿业集团总医院（以下简称“我院”）2014年1月～2016年1月住院治疗的AD患者35例作为AD组，选取同期在我院治疗的无中枢神经系统疾病、无痴呆的非神经系统疾病患者30例作为对照组。检测两组患者血清中超氧化物歧化酶（SOD）、丙二醛（MDA）、NO和一氧化氮合酶（NOS）水平及Aβ1-40和Aβ1-42的含量。 结果 AD患者组血清中MDA、NO及NOS水平明显高于对照组（P 0.05），SOD水平明显低于对照组（P 0.05）；AD患者组血清中Aβ1-40及Aβ1-42含量明显高于对照组（P 0.05）。Pearson相关性分析提示，AD患者血清中MDA、NO和NOS水平与血清Aβ含量呈正相关（P 0.05），而SOD水平与Aβ含量呈负相关（P 0.05）。 结论 动态检测AD患者体内血清中氧化应激相关指标及Aβ水平变化在AD临床诊疗中有着重要的意义，有利于了解病情发展状况、治疗效果及预后判断。 　　[关键词] 阿尔茨海默病；氧化应激；丙二醛；超氧化物歧化酶；一氧化氮；一氧化氮合酶；β-淀粉样蛋白 　　[中图分类号] R74 [文献标识码] A [文章编号] 1673-7210（2017）03（a）-0117-04 　　Clinical significance of oxidative stress related indexes and A-beta levels in the serum of patients Alzheimers disease 　　SHI Dianzhi 　　The Clinical Laboratory， General Hospital of Fushun Mining Group of China Medical University， Liaoning Province， Fushun 113008， China 　　[Abstract] Objective To study the clinical significance of oxidative stress related indicators and A-beta deposition related indicators in the serum of patients with Alzheimers disease （AD） in the process of diagnosis and treatment. Methods 35 cases patients with AD from General Hospital of Fushun Mining Bureau from January 2014 to January 2016 were selected as AD group， and 30 cases of non central nervous system disease and dementia non nervous system disease at the same hospital were selected as control group. SOD， MDA， NO and NOS levels and Aβ1-40 and Aβ1-42 content in the two groups were tested. The detection of SOD was tested by xanthine oxidase method， the detection of MDA was tested by thiobarbituric acid method， the detection of NO was tested by nitrate reductase method， the detection of NOS was tested by chemical colorimetry method and the detection of Aβ1-40 and Aβ1-42 was tested by ELISA. Results Compared with the control group， the MDA content in the serum of AD group was significantly higher （P 　　[Key words] Alzheimers disease； Oxidative stress； MDA； SOD； NO； NOS； Aβ