马兰叶中黄酮不同提取方法比较.docVIP

马兰叶中黄酮不同提取方法比较 　　摘要采用索氏提取法和纤维素酶辅助热浸提法提取马兰叶干粉中的黄酮,利用紫外分光光度法测定其提取液中黄酮含量分别为17.41 、30.60 mg/g,确定纤维素酶热浸提法适宜于提取马兰叶干粉中的黄酮,通过进一步的试验证实加标回收率为90.6%~93.6%,变异系数为0.05%~0.33%,结果较理想,精确度高。 　　关键词索氏提取法;纤维素酶辅助;热浸提法;马兰叶;黄酮;加标回收率;精密度 　　中图分类号TS201.2文献标识码A文章编号 1007-5739(2010)16-0021-01 　　 　　ComparisonofDifferentExtraction MethodsonFlavonoidsinKalimerisIndicaLeavesDetermination 　　JIANG Xian-guangHOU Dong-yanHUI Rui-huaDIAO Quan-pingGUO HuaWANG Yue-nanMA Li-jie 　　(Department of Chemistry,Anshan Normal University,Liaoming China,Anshan Liaoning 114005) 　　AbstractThe flavonoids in dried Kalimeris indica leaves powder were extracted by the soxhlet extraction method and the hot water extraction method with the help of cellulose enzyme. The content of flavonoids in their extracts were 17.41 mg/g and 38.80 mg/g respectively by Ultraviolet spectrophotometry,confirmed heat extraction method with cellulase enzyme auxiliary was suitable for the extraction of flavonoids in dried kalimeris indica leaves powder,by further tests,approved that the addition standard recovery was 90.6%~93.6%,the coefficient of variation was 0.05%~0.33%,the result was ideal,the precision was high. 　　Key wordssoxhlet extraction method;cellulase enzyme auxiliary;heat extraction method;the Kalimeris indica leaves;flavonoids;addition standard recovery;precision 　　 　　马兰(Kalimeris indica)为鸢尾科多年生草本植物,亦称泥鳅串、鸡儿肠、田边菊、路边菊、蓑衣莲、脾草、岗边菊、大风草等。马兰味苦、辛,性温、寒,以全草或根入药,有清热解毒,散瘀止血,温肺止咳,散寒止痛,消积之功效。主治感冒发热,咳嗽,急性咽炎,扁桃体炎,流行性腮腺炎,传染性肝炎,胃、十二指肠溃疡,小儿疳积,肠炎、痢疾、吐血、崩漏、月经不调、外用治疮疡肿毒、乳腺炎、外伤出血、肺寒喘咳、胃脘冷痛等[1];马兰全株入药,消炎止血,马兰花治痧症绞痛、无名肿毒、肝炎;幼苗作蔬菜[2]。马兰叶中含有黄酮类化合物,黄酮类化合物可预防与活性氧有关的动脉硬化、癌、糖尿病、帕金森氏病等疾病,有抗衰老作用,同时可调节毛细血管的脆性与渗透性,保护心脏,有抗溃疡、解毒、利尿、抗菌、消炎清热等功效[3-5]。为了更好地开发利用马兰叶,该文采用了2种不同的方法提取马兰叶中的黄酮,为其合理利用提供科学的理论依据。 　　1材料与方法 　　1.1试验材料 　　(1)原材料:新鲜马兰叶,采自辽宁鞍山。将马兰叶晒干,粉碎,过筛得马兰叶干粉,密封于干燥皿中贮存。 　　(2)主要试剂:芦丁(分析纯)、NaNO2(分析纯)、Al(NO3)3(分析纯)、NaOH(分析纯)、无水乙醇(分析纯)、纯净水。 　　(3)主要仪器:索氏提取器、紫外分光光度计、旋转蒸发仪。 　　1.2试验方法 　　(1)索氏提取法。称取2.0 g马兰叶干粉,置于索氏提取器中,加入150 mL 60%乙醇回流3 h,过滤,冷却,得粗提液,减压旋转蒸发,得黄酮浸